天津科技大学学报2011，Vol.26Issue(4)：6-9,4.
大肠杆菌L-组氨酸生物合成途径的改造及其对工程菌L-组氨酸产量的影响
Modification of L-histidine Biosynthesis Pathway in E.coli and Its Effect on L-histidine Yield
摘要
Abstract
To modify the metabolic pathway of L-histidine biosynthesis in E. Coli and increase L-histidine yield. E. Coli M-18 (SGr + 2-Tar + HisHxr) was obtained by N-Methyl-N-nitro-.N-nitrosoguanidine (NTG) mutagenesis derived from the original strain E. Coli M-17(SGr). The histidine operon was amplified by PCR from E. Coli M-18(SGr + 2-Tar + HisHxO chromosome and ligated it into the Puc118 vector. The recombinant plasmid Puc118-his-operon was transformed into E. Coli M-18 (SGr + 2-Tar + HisHxr) by electroporation. The zwf gene and prs gene were amplified by PCR, and then ligated to Pstv28 plasmid. The recombinant plasmids Pstv28-zwf, Pstv28-prs and Pstv28-zwf-prs were transformed into E. Coli M-19 (SGr + 2-Tar + HisHx/Puc118-his-operon), respectively. Flask-shaking fermentation results showed that, compared with E. Coli M-18, the L-histidine yield in the engineering strain E. Coli M-19 was 4.5 folds of that in the control, and in the two-plasmid system of recombinant engineering strains E. Coli MZH-19,E. Coli MPH-19 and E. Coli MZPH-19 were 5.14 folds, 5.78 folds and 8.43 folds of that in the control, respectively.关键词
L-组氨酸/组氨酸操纵子/大肠杆菌/双质粒系统/摇瓶发酵Key words
L-histidine/his operon/Escherichia coli/two-plasmid system/flask-shaking fermentation分类
生物科学引用本文复制引用
魏伟,刘倩,卢利宁,谢希贤..大肠杆菌L-组氨酸生物合成途径的改造及其对工程菌L-组氨酸产量的影响[J].天津科技大学学报,2011,26(4):6-9,4.基金项目
国家“十一五”科技支撑计划项目(2008BAI63B01) （2008BAI63B01）
