林业科学2011，Vol.47Issue(8)：88-94,7.
偏肿栓菌产锰过氧化物酶条件优化
Optimization of Ferment Conditions of Manganese Peroxidase Produced by Trametes gibbosa
摘要
Abstract
White rot fungus Trametes gibbosa was statically cultured at 28 ℃ in 4 kinds of different LNAS media. The solutions were sampled at different interval, and the optical density of the solutions, representing oxidized products of 2,6-DMP, ABTS and veratryl alcohol ( VA) catalyzed by manganese peroxidase (MnP) , laccase and lignin peroxidase (LiP) , were measured spectrophotometrically at 470 nm at 420 nm at 310 nm, respectively. Results showed that T. gibbosa could synthesize MnP (only when Mn2+ is present) and laccase (no matter Mn2+ is present or absent) simultaneously, but no LiP. Substrates wood sawdust and 2, 6-DMP could significantly enhance MnP and laccase activities. Furthmore, T. gibbosa was statically cultured at 30 ℃ in LNAS medium containing Mn1' and wood sawdust added was as initializing culture condition, and then two times orthogonal and several single-factor tests were conducted to optimize medium compositions and culture conditions of T. gibbosa for producing MnP. Results indicated that the most optimal culture condition within the fixed factors and levels was fructose 5 g·L-1,tartaric acid ammonium 15 mmol·L-1, Mn2+200 μmol·L-1, Tween -80 0.25 mL·L-1, MgSO4-7H2O 0.35 g·L-1, mineralt solution 10 mL·L-1, vitamin solution 1 mL·L-1 and pH 4. 5 in the improved LNAS medium; culture temperature 27 ℃ ; and 110 mL culture solution volume at 150 r·min shaking condition, and the produced manganese peroxidase activity could reach to 314.52 U·L-1, which was 8. 4 times higher than that produced at initializing culture condition. MnP yield produced by T. gibbosa could be enhanced in a great deal in the optimizing culture condition.关键词
偏肿栓菌/木质素降解酶/锰过氧化物酶/培养条件/正交试验Key words
Trametes gibbosa/ ligninolytic enzymes/ manganese peroxidase/ culture condition/ orthogonal test分类
农业科技引用本文复制引用
张玉龙,池玉杰,闫洪波..偏肿栓菌产锰过氧化物酶条件优化[J].林业科学,2011,47(8):88-94,7.基金项目
国家自然科学基金项目(30671700),中国博士后科学基金(20090460866),2009东北林业大学研究生科技创新项目. （30671700）
