山东农业大学学报（自然科学版）2011，Vol.42Issue(4)：477-482,6.
光谱法研究丙烯酰胺与牛血清白蛋白的相互作用
A STUDY OF THE INTERACTION BETWEEN ACRYLAMIDE AND BOVINE SERUM ALBUMIN USING SPECTROSCOPY METHODS
摘要
Abstract
The interaction between acrylamide (AA) and bovine serum albumin (BSA) was studied mainly from a spectroscopic angle. Under pH 7. 0, AA effectively quenched the intrinsic fluorescence of BSA via static quenching and conformational changes of BSA were observed. The number of binding sites (n =0. 933) and apparent binding constant (KA = 5.55 x 103 M-1) were calculated by relevant fluorescence data, and thus indicated the existence of just a single binding site in BSA for AA. According to the controlled experiments, the optimal reaction conditions were obtained as follows: the reaction time of 50 min, ionic strength of 0.05 mol·Kg-1, and pH =7.0. The effect of AA on the conformation of BSA can also be deduced by means of resonance scattering spectrum, UV - visible absorption spectrum and FT - IR measurement. Therefore, The binding study of AA with BSA is of great importance in the research of the effect of toxicity to biomolecules.关键词
丙烯酰胺/牛血清白蛋白/荧光猝灭/相互作用Key words
Acrylamide (AA) / BSA/ fluorescence quenching/ interaction分类
化学化工引用本文复制引用
鞠鹏,范海,吴晓娜,艾仕云,刘涛,崔琳..光谱法研究丙烯酰胺与牛血清白蛋白的相互作用[J].山东农业大学学报（自然科学版）,2011,42(4):477-482,6.基金项目
国家自然科学基金项目(21075078),山东省自然科学基金项目(ZR2010BM005) （21075078）
