中国水稻科学2011，Vol.25Issue(5)：467-474,8.DOI:10.3969/j.issn.1001-7216.2011.05.003
水稻microRNA单核苷酸多态性
Single Nucleotide Polymorphism of MicroRNA in Rice
摘要
Abstract
MicroRNAs (miRNAs) are a class of endogenous small (about 22 nucleotides), non-coding RNAs, which play important roles in plant growth, development and stress tolerance by binding to the target sites in mRNAs. Since a single nucleo-tide change in miRNAs can affect the maturity and regulation of miRNA, study on the distribution of single nucleotide polymorphisms (SNPs) in miRNA gene may be of great importance for functional and evolutionary research. In order to explore the potential functional significance of SNPs in the pre-miRNA in rice, we analyzed the distribution of SNPs in pre-miRNA region and flanking region, including 5' and 3' flanking regions in rice. The results showed that there was no significant difference in the frequency of SNPs between the pre-miRNA region and its flanking region in rice, which was different from the results in other species. Further study on the frequency of SNPs across pre-miRNA secondary structures showed that there was no significant difference between seed region and loop region, which was thought to undergo less negative selection pressure in other species. However, in consistent with other species, the frequency of SNPs in protein-coding gene region of rice was significantly lower than that in gene flanking region, indicating that the flanking region of pre-miRNA may be essential for the function of miRNA. Another reseaon was the uneven distribution of SNPs in different miRNAs. Some miRNAs were less important to rice function, thus was subjected to relaxation of selective pressure, accumulating more and more mutations to become a new gene or loss of functions. However, we could not rule out the bias in the results because of the unreliable data in current SNP database.关键词
microRNA/水稻/单核苷酸多态性Key words
microRNA/ rice/ single nucleotide polymorphism分类
生物科学引用本文复制引用
于颢,闫旭,郭卫东,辛德东..水稻microRNA单核苷酸多态性[J].中国水稻科学,2011,25(5):467-474,8.基金项目
浙江师范大学博士科研启动基金(ZC304009154). （ZC304009154）
