中国水稻科学2011，Vol.25Issue(5)：475-482,8.DOI:10.3969/j.issn.1001-7216.2011.05.004
稻米出饭特性QTL分析及遗传研究
Mapping and Genetic Analysis of Quantitative Trait Loci for Related Traits of Cooked Rice
摘要
Abstract
The milled rice length, cooked rice length and elongation traits are very important in the quality of cooked rice. Mapping of quantitative trait locus (QTL) for cooked rice related traits was conducted using a doubled haploid population from a cross between a typical indica rice cultivar *TN1' and a typical japonica rice cultivar 'Chunjiang 06'. Using a linkage map based on 177 SSR molecular marker loci covering the distance of 1670. 92 cM. a total of 14 QTLs were identified for the cooked rice related traits. One putative QTL for milled rice length and one for soaked rice length were mapped on chromosome 2, with the explained variance of 15. 20% and 18. 50% , respectively. One putative QTL for soaked rice expansion was mapped on chromosome 6, with the explained variance of 13. 39%. One putative QTL for boiled rice length was mapped on chromosome 9. With the explained variance of 13. 60%. Three QTLs for steamed rice length were detected on chromosomes 1. 3 and 12, totally explaining variance of 45. 00%. Four QTLs for boiled rice elongation were detected on chromosomes 3.6.9 and 10, totally explaining variance of 61. 30%. Three QTLs for steamed rice elongation were detected on chromosomes 1.3. And 6. Totally explaining variance of 49. 10%. Two QTLs for elongation related traits of cooked rice were detected on the Wx gene cluster and ALK gene cluster, respectively. Comparing to the W.r gene cluster, the QTL in the ALK gene cluster played a much more important role in regulation of related traits of cooked rice with the LOD score of 6. 35. These results provide a foundation for further cloning of genes that regulate cooked rice related traits and a theoretical basis for the improvement of rice cooking quality and marker-assisted breeding of high-yielding and high-quality rice varieties.关键词
水稻/出饭特性/数量性状基因座/加倍单倍体群体Key words
rice/ related traits of cooked rice/ quantitative trait locus/ doubled haploid population分类
农业科技引用本文复制引用
沈年伟,董国军,颜美仙,钱前,张光恒,来凯凯,粘金沯,曾大力,胡江,高振宇,郭龙彪,朱丽,刘坚..稻米出饭特性QTL分析及遗传研究[J].中国水稻科学,2011,25(5):475-482,8.基金项目
国家自然科学基金资助项目(30970171) （30970171）
国家转基因培育重大专项(2009ZX08009-125B) （2009ZX08009-125B）
浙江省杰出青年从金资助项目(R3100100) （R3100100）
浙江省科技计划资助项目(2009C32047). （2009C32047）
