中国动脉硬化杂志2011，Vol.19Issue(11)：891-896,6.
硫化氢对氧化型低密度脂蛋白诱导的人脐静脉内皮细胞凋亡的影响
Investigation on the Effect and Mechanisms of Hydrogen Sulfide Against Apoptosis Induced bv ox-LDL in Human Umbilical Vein Endothelial Cells
摘要
Abstract
Aim To investigate the effects of hydrogen sulfide on ox-LDL-induced apoptosis in human umbilical vein endothelial cells (HUVEC) and the mechanisms involved. Methods The HUVEC were incubated with ox-LDL for 24 hours after treatment with NaHS at different concentrations (25, 50, 100, 200 umol/L) for 24 hours or for different incubation time(6, 12, 24 hours) at the concentration of 50 ujnol/L. The apoptotic alterations of HUVEC were observed by Hoechst 33258 staining and flow cytometry (FCM) , respectively. The change of mitochondrial membrane potential (MMP) and intracellular reactive oxygen species(ROS) generation were detected by Dihydrohodaminel23 (DHR123) staining and rhodaminel23 (Rhl23) staining. Results Pre-treatment with NaHS remarkably inhibited apoptosis induced by ox-LDL in HUVEC in a time- and concentration-dependent manner( all P <0. 01). The decrease of MMP and increase of the intracellular ROS generation in HUVEC induced by ox-LDL were significantly prohibited by treatment with NaHS or N- acetyl-L-cysteine (NAC) (all P <0.05). Conclusions H2S inhibits apoptosis induced by ox-LDL in HUVEC which is involved in preservation of mitochondrial membrane potential and attenuation of the intracellular ROS generation.关键词
硫化氢/氧化型低密度脂蛋白/N-乙酰半胱氨酸/人脐静脉内皮细胞/凋亡/细胞线粒体膜电位/细胞内活性氧Key words
Hydrogen Sulfide/ ox-LDL/ NAC/ HUVEC/ Apoptosis/ Mitochondrial Membrane Potential/Reactive Oxygen Species分类
医药卫生引用本文复制引用
任重,赵战芝,彭湘萍,谢巍,刘艳文,索荣,姜志胜..硫化氢对氧化型低密度脂蛋白诱导的人脐静脉内皮细胞凋亡的影响[J].中国动脉硬化杂志,2011,19(11):891-896,6.基金项目
国家自然科学基金项目(30971169)和衡阳市科学技术发展计划项目(2009KJ1O) （30971169）
