组织工程与重建外科杂志2011，Vol.7Issue(5)：241-243,3.DOI:10.3969/j.issn.1673-0364.2011.05.001
应用细胞膜片复合硅胶管内支撑构建组织工程管状软骨
Construction of Scaffold-Free Cylindrical Cartilage Combined with Cell Sheet and Silicon Tube
摘要
Abstract
Objective To explore the feasibility of fabricating a scaffold-free cylindrical cartilage using cell sheet with silicon tube as interior support in vivo. Methods Auricular chondrocytes were harvested from goat and the second passage of auricular chondrocytes were cultivated with high-density of 2.0×106 cells/mL to form a chondrocyte sheet with the diameter of 35 mm. Then the silicon tube covered with cell sheet was implanted into the back of nude mice. The tissue engineered cylindrical cartilages were evaluated by gross observation, histological and immunohistochemical examination. Results The cartilage was sufficiently elastic and stiff to maintain the structure without disruption. Cartilage tissue was observed by HE staining, Safranin O staining and immunohistochemical staining for type II collagen. Conclusion A novel technique was developed for fabricating engineered cylindrical cartilage using cell sheet with silicon tube in vivo.关键词
无细胞支架/细胞膜片/管状软骨/气管软骨Key words
Scaffold-free/ Cell sheet/ Cylindrical cartilage/ Tracheal cartilage分类
生物科学引用本文复制引用
王健,刘霞,肖苒,曹谊林..应用细胞膜片复合硅胶管内支撑构建组织工程管状软骨[J].组织工程与重建外科杂志,2011,7(5):241-243,3.基金项目
北京市科委科技计划重大项目资助(D090800046609003),卫生部部属医院临床学科重点项目,国家自然科学基金青年科学基金项目(30801192),北京市自然科学基金(7102134),高等学校博士学科点专项科研基金(新教师基金课题,200800231078). （D090800046609003）
