四个主要产地广藿香HPLC指纹图谱比较分析OA北大核心CSCDCSTPCD
Analyze on HPLC Fingerprint of Four Main Metropolis of Herba Pogostemonis
目的:建立广藿香HPLC指纹图谱的分析方法,对四个主要产地的广藿香指纹图谱进行比较,为广藿香药材内在质量评价积累数据.方法:色谱柱:ODS色谱柱Diamonsil (R)C18(250 mm×4.6mm,5μm)；流动相:甲醇-0.1％磷酸水溶液梯度洗脱；检测波长:254 nm；柱温:25℃；流速:1.0mL/min.结果:四个不同产地的广藿香指纹图谱存在一定的差异,其中广州黄村和肇庆高要的药材相似度大于0.9,而这两地与湛江吴川和海南万宁比较差异较大.结论:方法准确可靠,重复性好,为广藿香药材道地性、真伪鉴别及品质评价提供了参考依据.
Objective: To establish a method for analyzing HPLC fingerprint of Herba pogostemonis and compare the variability of four main producing areas. Methods; Diamonsil(R) C18 column was used, the Methanol-0. 1% phosphoric acid (gradient elution)as a mobile phase and detection wavelength was set at 254nm,column temperature was 25℃. And flow rate was 1.0 mL/min. Results; There were differences between the HPLC fingerprint of Herba pogostemonis from various places of production. The similarity of Herba Pog- . Ostemonis cultivated in tow regions of Zhaoqing Caoyao and Guangzhou Huangcun was over 90% , and there were more differences a-mong them with Zhanjiang Wuchuan and Hainan Wanning. Conclusion:The method is reliable and accurate. The method can be used for the identification of the crude drug and the evaluation of its quality.
黄木土;罗集鹏
广州军区广州总医院药学部,广东广州510010广东药学院中药学院,广东广州510006
医药卫生
广藿香HPLC指纹图谱产地
Herba PogostemonisHPLC fingerprintProducing area
《中药材》 2011 (10)
1521-1524,4
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