中华耳科学杂志2011，Vol.9Issue(3)：269-271,3.
豚鼠耳蜗盖膜下面的超微结构
Ultrastructure of undersurface of tectorial membrane in Guinea pigs
摘要
Abstract
Objective To demonstrate infrastructure of the undersurface of the tectorial membrane using scanning electron microscopy (SEM) to advance understanding of mechanismsin cochlear sound reception. Methods Tectorial membranes from 12 cochleae in 6 guinea pigs were studied under a HITACHI S-4800 scanning electron microscope. Results (1) Imprints from thestereocilia of outer hair cells were clearly visible on the undersurface of the tectorial membrane, seemingly corresponding to tips of the tallest row of stereocilia. The imprints were "W" shaped in the basal turn, but gradually turned into "V" or irregular shapes towards the apical turn. The radiant fibers on the undersurface were interrupted by the imprints which appeared as a string of beads on the undersurface. (2) A deep linear groove was seen at locations corresponding to inner hair cells, which appeared shallower but broader than outer hair cell imprints. The groove widened from the basal to apical turn. (3) The fibers on the undersurface of the tectorial membrane were radially arranged between theouter edge of the tectorial membrane and inner hair cell stereocilia imprints and interrupted by outer hair cell stereooilia imprints. These were fine fibers, interlaced and connected with each other. Fibers between inner and outer hair cell imprints (I.e., areas over pillar cells) were especially thin and uniform looking filaments, whereas those fibers between inner hair cell imprints and the spiral limbus (above the inner sulcus) appeared even finer and longer. Conclusion The imprints on the undersurface of tectorial membrane clearly indicate contact between the membrane and hair cell stereocilia. The size and morphological differences between fibers at different locations may indicate a role of the tectorial membrane in mechanical tuning.关键词
豚鼠/盖膜/静纤毛压迹/超微结构/扫描电镜Key words
Guinea pig/ Tectorial membrane/ Stereocilia print/ Ultrastructure/ SEM分类
医药卫生引用本文复制引用
郭维维,孙建和,杨仕明..豚鼠耳蜗盖膜下面的超微结构[J].中华耳科学杂志,2011,9(3):269-271,3.基金项目
国家973计划重大科学问题导向项目(2011CBA01000) （2011CBA01000）
国家863计划(2007AA02Z150) （2007AA02Z150）
国家自然科学基金重点项目(30730040)和面上项目(30871398) （30730040）
国家十一五科技支撑计划重大项目(2008BAI50B08,2007BAI18B12,2007BAI18B14). （2008BAI50B08,2007BAI18B12,2007BAI18B14）
