中华耳科学杂志2011，Vol.9Issue(3)：244-248,5.
E2F2过表达使内耳感觉细胞重新进入细胞周期
Re-entry into cell cycles induced by E2F2 over-expressionin inner ear sensory cells
摘要
Abstract
Objective To induce re-entry into cell cycles in cochlear terminal stationary cells via over expression of theE2F2 gene in adult mammals, for the purpose of increasing the number of hair cells. Methods Full-length human E2F2 gene was constructed into adenovirus vectors and introduced into the cochlea through the round window. Morphology of cochlear cells was studied using whole-mount and slice cochlear preparations. Cellular proliferation was detected with BrdU labeling and ki67 staining. Results E2F2 gene expression was seen in cochlear hair cells and supporting cells, as well as in spiral ganglion cells. Following E2F2 expression, there were also ki67- and BrdU-positive cells in the cochlea. Conclusion Over-expression of E2F2 appears to allow end stage cells to re-enter the cell cycle. This technique may represent a promising new way of cell regeneration.关键词
E2F2/细胞周期再进入/耳蜗/毛细胞Key words
E2F2/ Cell cycle re-entry/Cochlea/Hair cells分类
医药卫生引用本文复制引用
郭维维,王琳,余力生,杨仕明..E2F2过表达使内耳感觉细胞重新进入细胞周期[J].中华耳科学杂志,2011,9(3):244-248,5.基金项目
国家973计划重大科学问题导向项目(2011CBA0 1000) （2011CBA0 1000）
国家863计划(2007AA02Z 150) （2007AA02Z 150）
国家自然科学基金重点项目(30730040)和面上项目(30871398) （30730040）
国家十一五科技支撑计划重大项目(2008BAI50B08,2007BAI 18B 12,2007BAI18B14). （2008BAI50B08,2007BAI 18B 12,2007BAI18B14）
