西北林学院学报2011，Vol.26Issue(6)：119-123,5.
DPPH·法评价杜仲叶提取物的抗氧化活性
Evaluation of Antioxidant Activity of Eucommia ulmoides Leaf Extracts by DPPH · Assay
摘要
Abstract
The antioxidant components of Eucommia ulmoides leaves were extracted by ultrasonic-assisted method and their antioxidant activities were investigated by scavenging DPPH ? . The extracts obtained from optimum technological conditions were further extracted by different polar organic solvents and the contents of total phenolic compounds, total flavonoids and DPPH radical scavenging activity were determined. The results showed that the optimal parameters of the ultrasonic-assisted extraction were as follows s ethanol concentration 60%, solid to liquid ratio 1 ? 15, temperature §Q°Q. And extracting time 30 min. Under these conditions, the clearance rate to DPPH ? Were 91. 01%. The bioactive antioxidants of E. Ulmoides leaves were mainly attributed to the ethyl acetate fraction. The percentage of scavenging DPPH radical of the sample of the ethyl acetate fraction with the concentration of 100 fig ? mL"1 was 92. 28% , stronger than those of BHT (/><0. 05) and no significant differences compared with those of Vc. The result of correlation analysis showed that the strong antioxidant activity mainly resulted from the high contents of the total phenols and total flavonoids.关键词
杜仲叶/抗氧化活性/提取工艺/DPPH·/总多酚/总黄酮Key words
Eucommia ulmoides leaves/ antioxidant activity/ extracting technology/ DPPH ? / total phenols/ total flavonoids分类
农业科技引用本文复制引用
苑子夜,苏印泉,张强,朱铭强,中泽庆久,堤雅史..DPPH·法评价杜仲叶提取物的抗氧化活性[J].西北林学院学报,2011,26(6):119-123,5.基金项目
中日合作课题(K332020908) （K332020908）
陕西省f日元贷款项目(K332020023) （K332020023）
