中华耳科学杂志2011，Vol.9Issue(3)：249-253,5.
DAPT对离体培养的基底膜的影响
In vitro effects of DAPT on Organ of Corti
摘要
Abstract
Objective To study hair cell changes in new born rats when the Notch signal is inhibited by DAPT in vitro. Methods Cochleae were dissected from new born rats. The Organ of Corti was freed from surrounding tissues and explanted inextenso onto the surface of 6 well culture plates and randomly divided into two groups: an experiment group in which specimens were treated with DAPT (a Notch signal inhibitor) at 5 μM, and a control group in which specimens were treated with DMEM containing DMSO with a final concentration lower than 0.1%. The Organ of Corti wag incubated at 37 degree in a humidified atmosphere with 5% CO2 for 5 days with daily replacement of the culture medium. The number of hair cells and morphology of the basement membrane were then examined after immunohistochemical staining. RT-PCR was used to confirm presence of Notch signal on P0, and quantitative RT-PCR was used to determine changes in downstream genes involved in Notch signaling on PS. Results There was an increase of hair cells in the experiment group compared to the control group. Notch signaling was present on P0 but Notch signaling genes changed after DAPT treatment. Conclusions Notch signaling is present in new born mammals. Increasing hair cells may be possible through inhibiting Notch signaling.关键词
DAPT/Notch通路/内耳毛细胞/大鼠/RT-PCRKey words
DAPT/ Notch signaling/Hair cells/Rats/RT-PCR分类
医药卫生引用本文复制引用
李新新,杨仕明,郭维维,邢光前..DAPT对离体培养的基底膜的影响[J].中华耳科学杂志,2011,9(3):249-253,5.基金项目
国家973计划重大科学问题导向项目(2011CBA01000) （2011CBA01000）
国家863计划(2007AA02Z 150) （2007AA02Z 150）
国家自然科学基金重点项目(30730040)和面上项目(30871398) （30730040）
国家十一五科技支撑计划重大项目(2008BAI50B08,2007BAI18B12,2007BAI 18B14) （2008BAI50B08,2007BAI18B12,2007BAI 18B14）
江苏省“科教兴卫工程”医学重点人才基金(RC2007064). （RC2007064）
