抗谷胱甘肽-S-转移酶(GST)单克隆抗体的研制及初步应用OA北大核心CSCDCSTPCD
Preparation of Monoclonal Antibodies against Glutathione-S-transferase (GST)
以纯化的谷胱甘肽转移酶标签蛋白(GST)免疫Balb/C小鼠,取免疫鼠脾细胞与NSO骨髓瘤细胞按常规方法融合,用纯化的GST经间接ELISA筛选,阳性孔经3次有限稀释法亚克隆,最终获得2株抗GST的单克隆杂交瘤细胞株,分别命名为4B7-F4和4B7-F4.间接ELISA检测4B7-E4、4B7-F4细胞培养上清的效价分别为1∶4000、1∶3000,腹水效价分别为1∶3×106和1∶2×106.Western-blot结果表明,这两株单抗均可以特…查看全部>>
The monoclonal antibodies (McAbs) against Glutathione-S-transferase (GST) were prepared in the present work. Balb/C mice were immunized with purified GST and the cell fusion was performed by standard method. The hybridom secreting antibodies were subcloned three times by limiting dilution. Two hybridomas, which were named as 4B7-E4 or 4B7-F4 and secreted McAbs against GST, were picked out by indirect ELISA. The ELISA antibody titers of cell culture sup…查看全部>>
滕蔓;罗俊;王秋霞;王丽;张改平
河南省农业科学院,农业部动物免疫学重点开放实验室,河南省动物免疫学重点实验室,河南郑州450002河南省农业科学院,农业部动物免疫学重点开放实验室,河南省动物免疫学重点实验室,河南郑州450002河南省农业科学院,农业部动物免疫学重点开放实验室,河南省动物免疫学重点实验室,河南郑州450002河南省农业科学院,农业部动物免疫学重点开放实验室,河南省动物免疫学重点实验室,河南郑州450002河南省农业科学院,农业部动物免疫学重点开放实验室,河南省动物免疫学重点实验室,河南郑州450002
生物科学
GST单克隆抗体间接ELISA
GSTMcAbIndirect ELISA
《华北农学报》 2011 (4)
82-85,4
国家公益性行业(农业)科研专项经费资助(200803015)
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