中国临床医学2011，Vol.18Issue(5)：587-590,4.
人血管内皮生长因子165基因联合间充质干细胞治疗对扩张型心肌病胶原重塑的影响
Collagen Remodeling Effect of Transplantation Mesenchymal Stem Cells with a Cardiac-Specific Expressing Human Vascular Endothelial Growth Factor-165 Gene in Dilated Cardiomyopathy Animal Model
摘要
Abstract
Objective:To investigate the collagen remodeling effect of transplantation mesenchymal stem cells (MSCs) combined with a cardiac-specific expressing human vascular endothelial growth factor-165 (hVEGF165) gene in dilated cardiomyopathy (DCM) animal model. Methods: The bone marrow-derived mesenchymal stem cells of rat were isolated, cultured and expanded ex vivo. The characteristics of those cells were identified by flow cytomery. The subjects were divided into PBS group, MSCs transplantation group (MSCs), gene therapeutic group (GENE), and MSCs transplantation combined with gene transfection group (MSCs-GENE), in which the MSCs and/or pmlc-2v-EGFP-hVEGF165 were injected into the rats' heart. After 1 month, reverse transcriptase PCR (RT-PCR) wasused to detect the mRNA expressions of type I, type III collagen and human transforming growth factor β1 ( TGF-β1). The expressions of hVEGF165 were documented by Western blot. Results; Although the optical densities of TGF-β1 were significantly lower in MSCs-GENE group and GENE group than those in PBS group and MSCs, there were no difference between MSCs-GENEgroup and GENE group (P = 0.910), which suggested the expressions of TGF-β1 were down-regulation in both MSCs-GENE group and GENE group. Comparison of optical density ratio of type I/HI collagen, the optical densities ratios were lower in MSCs-GENE group than that in MSCs group and GENE group, but there was no statistics different (P = 0.172). Though there were expressions of GAPDH in each group, but the expression of hVEGF165 were detected in MSCs-GENE group and GENE group, which suggested the target gene specifically expressing its products in myocardial. Conclusions: The mechanism of cardiac function improved by MSCs with VEGF genes may be related to the down-regulation of TGF-β1 expression, decreasing ratio of type I/III collagen, and increased myocardial compliance and attenuation of remodeling of myocardial collagen lattice.关键词
人血管内皮生长因子165基因/胶原重塑/转化生长因子β1/间充质干细胞/基因治疗/扩张型心肌病Key words
Human vascular endothelial growth factor 165 genet Collagen remodeling/Transforming growth factor β1/Mesenchymal stem cells/Gene therapy/Dilated cardiomyopathy分类
医药卫生引用本文复制引用
于勤,朱宁,李倩晓,李晓菲,那荣妹,郑晓群,方唯一..人血管内皮生长因子165基因联合间充质干细胞治疗对扩张型心肌病胶原重塑的影响[J].中国临床医学,2011,18(5):587-590,4.基金项目
辽宁省教育厅高等学校科研计划项目(编号:L2010023) （编号:L2010023）
大连市科技局科研计划项目(编号:2007E21SF203) （编号:2007E21SF203）
