中国临床医学2011，Vol.18Issue(5)：591-594,4.
结直肠癌前哨淋巴结活检及前哨淋巴结微转移检测方法研究
Priliminary Study of Sentinel Lymph Node Biopsy in Colorectal Cancer and Detection of Micrometastasis in Sentinel Lymph Node
摘要
Abstract
Objective:To evaluate the feasibility of sentinel lymph node (SLN) biopsy with carbon nanoparticles (CNP) and 99mTc sulfur colloid (TSC) for colorectal cancer. To evaluate the value of immunohistochemistry (IHC) and quantitative Real Time PCR (QRT-PCR) for detection micrometastasis in SLN. Methods: Twenty-two patients who underwent radical resection of a primary colorectal cancer were enrolled. During operation, SLN mapping was performed . AH lymph nodes were stained with hematoxylin eosin (HE) staining. SLNs, when negative by routione HE staining, were further analyzed with CK20 IHC staining. SLN tissue from 20 patients were analyzed with qRT-PCR. Results: SLNs were detected in 20 of 22 patients with a successful detection rate of 90. 91 %. The number of SLNs ranged from 1 to 4, with a mean value of 1. 75 ± 0. 97 per case. Of 10 patients with SLNs being negative by HE staining, there were 2 patients detected micrometatastases by CK20 IHC. Five of twenty patients were found high level CK20-mRNA in SLN tissue by qRT-PCR. Conclusions: Combined-agent SLN mapping is a potential diagnostic method for predicting local lymph node metastasis in the patients with CRC. IHC and qRT-PCR can increase detection rate of micrometastasis in SLN. But the clinical value of difference of CK20-mRNA in SLN needs to be further studied.关键词
结直肠肿瘤/前哨淋巴结/微转移/实时定量聚合酶链反应/免疫组织化学Key words
Colorectal neoplasms/Sentined lymph node) Micrometastasis/Quantitative RT-PCR/Immunohistochemistry分类
医药卫生引用本文复制引用
童汉兴,张勇,张思,陆巍,江颖,朱隽,陆维祺..结直肠癌前哨淋巴结活检及前哨淋巴结微转移检测方法研究[J].中国临床医学,2011,18(5):591-594,4.基金项目
上海市长宁区卫生局基金资助项目(编号:20054Y21001) （编号:20054Y21001）
