四川大学学报（医学版）2011，Vol.42Issue(6)：757-761,788,6.
TS-Fe3O4介导丙型肝炎病毒多表位基因疫苗的研究
Investigation of HCV Multi-epitope Gene Vaccine Loaded by CTS-Fe3O4
摘要
Abstract
Objective In order to develop a promising HCV gene vaccine candidate to induce effective immune response and explore the application of magnetic nanoparticles as gene delivery system. Methods The DNA fragment containing multi-epitope antigen gene of HCV with five conserved mimotopes was synthesized and cloned into plasmid pcDNA3. 1 ( + ). The Fe3O4 modified with chitoson was prepared and the cytotoxicity of the magnetic material was detected in vitro. Analysis of recombinant plasmid in vitro expression, and its immunogenicity loaded by CTS-Fe3O4, in mice were evaluated in detail. Results The HCV multi-epitope gene vaccine pcDNA3. 1 (+)-MA was successfully constructed and recognized by 81% HCV positive sera. There was no cytotoxicity of CTS-Fe3O4 when its concentration was equal or less than 1 mmol/L. Both the antibody production and T-cell activity were induced. Conclusion It was believed that DNA encoding MA was an attractive approach for the therapeutic and prophylactic vaccines against HCV and the Fe3O4 modified with chitoson showed excellent target, safety and adjuvant effect as gene carrier.关键词
丙型肝炎病毒/多表位基因/磁性纳米材料/壳聚糖/基因投递系统Key words
HCV/ Multi-epitope gene/ Magnetic nanoparticles/ Chitoson/ Gene delivery system引用本文复制引用
杨远,邝玉,袁敦,曹丽丽,王保宁,李明远,张中伟..TS-Fe3O4介导丙型肝炎病毒多表位基因疫苗的研究[J].四川大学学报（医学版）,2011,42(6):757-761,788,6.基金项目
国家自然科学基金(批准号30901270)资助 （批准号30901270）
