四川大学学报（医学版）2011，Vol.42Issue(6)：784-788,5.
吡格列酮对缺氧/复氧后新生大鼠心肌细胞线粒体膜电位的影响
Effects of Pioglitazone on Mitochondrial Membrane Potential of Neonate Rat's Myocardial Cells after Hypoxia/ Reoxygenation
摘要
Abstract
Objective To explore the effectiveness and mechanism of pioglitazone on mitochondrial membrane potential of neonate rat's myocardial cells after hypoxia/reoxygenation. Methods Primary cultured myocardial cells of neonate Sprague-Dawley rats were pretreated with different concentrations of pioglitazone, pioglitazone' s inhibitor and Chelerythrine, an inhibitor of protein kinase C (PKC). Hypoxia/reoxygenation model was established after 24 h of pretreatment. Subsequently, the mitochondrial membrane potential was detected with JC-1 staining under laser confocal microscopy during reoxygenation phase. Results Compared with the control group, red/green fluorescent ratio of myocardial cells in the H-r model group decreased significantly after hypoxia/reoxygenation (red/green fluorescent ratio reduced from 1. 20±0. 05 to 1. 13±0. 02,P<0. 01 ), and also decreased in 0. 1 μmol/ L, 1 μmol/L and 2 μmol/L pioglitazone group (1. 11 + 0. 01, 1. 10±0. 01, 1.16 + 0.03, P<0. 01, respectively). The red/green fluorescent ratio in pioglitazone + GW9662 group (1. 12 ± 0. 02) and pioglitazone + Chelerythrine group (1. 07 ± 0. 01) were both significantly lower than those in 2 μmol/L pioglitazone group (P<0. 01). Conclusion The mitochondrial membrane potential of neonate rat' s myocardial cells was descend after hypoxia/ reoxygenation, while pioglitazone can interrupt the process, indicating that the activation may be relevant to peroxisome proliferator-activated receptor gamma (PPARγ) ligand and PKC pathway.关键词
过氧化物酶体增殖物激活型受体γ/线粒体膜电位/激光共聚焦扫描显微镜Key words
Peroxisome proliferator-activated receptor gamma (PPARγ)/ Mitochondrial membrane potential/ Laser confocal microscopy引用本文复制引用
朱启伟,王浩,叶平,骆雷鸣,张今尧..吡格列酮对缺氧/复氧后新生大鼠心肌细胞线粒体膜电位的影响[J].四川大学学报（医学版）,2011,42(6):784-788,5.基金项目
国家自然科学基金(批准号30872713)和北京市自然科学基(7082083)资助 （批准号30872713）
