山东医药2011，Vol.51Issue(39)：22-24,3.
天然药物IHA-01筛选敏感肿瘤细胞株及其对结肠癌细胞增殖的影响
Screening of sensitive tumor cells of traditional Chinese medicine IHA-01 and its effects on the proliferation of colon carcinoma cells
摘要
Abstract
Objective To screen out sensitive tumor cell lines with traditional Chinese medicine IHA-01, and study its anti-tumor mechanism. Methods Twelve strains of human cancer cell lines were cultured in vitro, then treated with IHA-01 at 5 different concentrations for 48 hours. Cell morphology changes were observed by microscope, and IC50 (half inhibitory concentration) values was obtained by CCK-8 assay to confirm the sensitive cell line; the apoptosis and telomerase activitieof HCT-8 exposed to the optimal concentration of IHA-01 were detected by flow cytometry. Results Five different concentrations of IHA-01 inhibited the proliferation of 12 strains human cancer cell lines, in which the sensitivity of colon cancer cells HCT-8 was the highest, the optimal concentration was 1.29 μg/ml; the CNE-2 cell was not sensitive. The apoptosis rate and telomerase activity of HCT-8 cell were respectively higher and lower than those of CNE-2 cell after treated with IHA-01 of 1.29 μg/ml (both P <0.01). Conclusions The IHA-01 can inhibit the HCT-8 cell strongly; the mechanism maybe associated with inducing tumor cells apoptosis and inhibiting telomerase activity.关键词
IHA-01/结肠癌细胞/细胞凋亡/端粒酶活性Key words
IHA-01/colon carcinoma cells/apoptosis/telomerase activity分类
医药卫生引用本文复制引用
李秋恬,洪敏,陈晓,李少遊,缪延栋,耿计伟,李臣,尹燕鹰,董坚..天然药物IHA-01筛选敏感肿瘤细胞株及其对结肠癌细胞增殖的影响[J].山东医药,2011,51(39):22-24,3.基金项目
云南省科技计划重点新产品开发计划项目(2007BC002) （2007BC002）
云南省科技计划条件平台建设项目(2007 DA006). （2007 DA006）
