激光生物学报2011，Vol.20Issue(5)：619-623,5.DOI:10.3969/j.issn.1007-7146.2011.05.010
辐照地被竹突变体DNA多态性分析
DNA Polymorphic Analysis of Irradiated Dwarf Bamboo Mutants
摘要
Abstract
Genomic DNA variation of dwarf bamboo mutants by the irradiation of 137Cs γ-ray using ISSR were analysed and detected. The results showed that the 21 high polymorphic, good repeatable primers were screened from 100 ISSR primers. The effect of ISSR polymorphism were that the amplification parts increased and reduced and the difference of length of amplification parts. The rates of polymorphism from different mutants were 19.46%~72.39% , which showed that the frequency of mutation of genetic material in different mutants, which laid the foundation for the establishment of special dwarf bamboo germ plasm resource.关键词
辐照/地被竹/ISSR/多态性Key words
irradiation/dwarf bamboo/ISSR/polymorphism分类
生物科学引用本文复制引用
白瑞华,丁兴萃,王树东,邵琼,毕毓芳..辐照地被竹突变体DNA多态性分析[J].激光生物学报,2011,20(5):619-623,5.基金项目
中国林科院林业新技术所所长基金项目(CAFINT2008C 12) （CAFINT2008C 12）
