山东医药2011，Vol.51Issue(43)：1-3,3.
高浓度葡萄糖条件下罗格列酮对NIT-1细胞增殖、胰岛素分泌水平及IRS-2表达的影响
Effects of rosiglitazone on proliferation of NIT-1 cells, secretion level of insulin and expression of IRS-2 under condition of high concentration glucose
摘要
Abstract
Objective To investigate the effects of rosiglitazone on cell proliferation of pancreatic β cells and insulin secretory function, under condition of high concentration glucose. Methods The pancreatic β cells NIT-1 in logarithmic phase were digested and centrifuged, then were put into plates (5 x 104 cells/well) and cultivated for 48 h and treated with different concentrations of glucose at 5.6-27.6 mmol/L. After cultivated for 24 hs, all the cell were divided randomly into control group and rosiglitazone 1-3 groups, and were interfered with rosiglitazone of 0-10-5 mol/L, then the supernatant was collected aftert 24 and 48 hs, the cell proliferation were measured by MTT techniques. Insulin level was evaluated by radio-immunity assay. The expression levels of 1RS-2 protein were detected by Western blot respectively. Results ①Cell proliferation and insulin secretion of the four groups decreased as glucose levels elevated, the cell proliferation and insulin secretion of rosiglitazone 1-3 groups were significantly higher than those of the control group (P <0.05,0.01). ②The insulin secretion levels in rosiglitazone 3 group were significantly higher than those in the control group, under the condition of 22.5, 27.6 mmol/L glucose for 24 hs and 11.1 mmol/L for 48 hs( P <0.05, 0.01) ; under the condition of different glucose concentration, the expression of IRS-2 protein in rosiglitazone 3 group were higher than those in control group (P < 0.05, 0.01), and it varied with glucose concentration change trend of the same as above. Conclusion Under high glucose concentration condition, rosiglitazone can improve the secretion function and cell proliferation of pancreatic β cells, the mechanism maybe related to the up-regulation of IRS-2 expression.关键词
高浓度葡萄糖/胰岛β细胞/胰岛素受体底物-2/罗格列酮Key words
glucose of high concentration/pancreatic β cells/insulin receptor substrate-2/rosiglitazone分类
医药卫生引用本文复制引用
于冉冉,乔伟,梁瑜祯,冯乐平..高浓度葡萄糖条件下罗格列酮对NIT-1细胞增殖、胰岛素分泌水平及IRS-2表达的影响[J].山东医药,2011,51(43):1-3,3.基金项目
国家自然科学基金资助项目(30860116、31060161) （30860116、31060161）
广西壮族自治区教育厅科研基金资助课题项目(201010LX325、201010LX326). （201010LX325、201010LX326）
