山东医药2011，Vol.51Issue(43)：4-5,2.
EV71VP1黏膜疫苗制备及其诱导黏膜免疫的实验研究
Preparation and experimental study of EV71 VP1 mucosa vaccine in mucosal immune response
摘要
Abstract
Objective To study the feasibility and value of bacillus subtilis spores which displayed VP1 of EV71 on surface as vaccine. Methods To prepare vaccines with bacillus subtilis spores displaying VP1 and wild-type isogenic strain, then separately immuned mice orally and intranasally (16 mice in observed group and control group separately), the eyeballs were picked out to get blood 4 weeks later. The mice were killed by cervical dislocation to collect flushing fluids of pulmonary alveolar and small intestine, then the VP1-specific IgA in serum, lung and intestine were detected by ELISA. Results Bacillus subtilis formed spores after treated with muramidase. VP1 -specific IgA in serum and lung of observed group were significantly higher than those of the control group(both P<0.01), but there was no difference of VP1-specific IgA in intestine between the two groups. Conclusion The spores displaying EV71 VP1 can be prepared into vaccine and elicit special mucosal immune response; this study provide a new way in the filed of EV71 vaccine.关键词
肠道病毒71型/衣壳蛋白VP1/疫苗/枯草芽孢杆菌芽孢Key words
enterovirus 71/capsid protein VP1/vaccine/bacillus subtilis spores分类
医药卫生引用本文复制引用
李志会,岳盈盈,李鹏,宋楠楠,孟红..EV71VP1黏膜疫苗制备及其诱导黏膜免疫的实验研究[J].山东医药,2011,51(43):4-5,2.基金项目
国家自然科学基金资助项目(81000720) （81000720）
山东省自然科学基金资助项目(2009ZRC03096). （2009ZRC03096）
