化工学报2011，Vol.62Issue(10)：2876-2884,9.DOI:10.3969/j.issn.0438-1157.2011.10.028
聚乙二醇修饰重组人干扰素ω的过程优化
Processing parameters optimization of PEGylation of recombinant human interferon omega
潘红春 1刘红 1程永刚 2彭力 2徐波 2杨红涛2
作者信息
- 1. 西南大学药学院,重庆400716
- 2. 太极集团西南药业股份有限公司,重庆400038
- 折叠
摘要
Abstract
In order to reduce the antigenicity and enhance the stability of the recombinant human interferon omega (rhIFNω) expressed in Pichia pastoris, the amino group modification of rhIFNco with mono-methoxy polyethylene glycol succinimidyl succinate (mPEG-SS, 20000) was investigated.lt was found that the optimized reaction conditions for the highest modification yield of mono PEG-rhlFNto were as follows: in 50 mmol ? L-1 and pH 8. 5 phosphate buffer, concentration of rhIFNco 0. 75 mg ? Ml-1, molar ratio of rhlFNco to PEG 1 ∶ 7, and reaction time 4 h at 251. Under the optimized conditions, the content and modification yield of mono PEG-rhlFNω reached 0.25 mg ? Ml-1 and 34.63%, respectively. The purified PEG-rhlFNco had the characteristics of typical PEGylated protein. The experiments showed that the antiviral activity of prepared PEG-rhlFNco could retain about 15% of the antiviral activity of the native rhIFNω.关键词
重组人干扰素ω/聚乙二醇/过程优化/抗病毒活性Key words
recombinant human interferon omega (rhIFNco)/ PEGylation/ process optimization/ antiviral activity分类
化学化工引用本文复制引用
潘红春,刘红,程永刚,彭力,徐波,杨红涛..聚乙二醇修饰重组人干扰素ω的过程优化[J].化工学报,2011,62(10):2876-2884,9.
