中国中医药信息杂志2011，Vol.18Issue(11)：65-66,100,3.DOI:10.3969/j.issn.1005-5304.2011.11.026
黄芪、 参、三七中多糖提取工艺考察
Extraction of Crude Polysaccharide from Radix astragali, Radix salviae miltiorrhizae and Radix notoginseng
摘要
Abstract
Objective To investigate the technological parameters of extracting crude polysaccharide from the mixture dregs of a decoction containing Radix astragali, Radix salviae miltiorrhizae and Radix notoginseng. Methods Orthogonal experiment was used to evaluate the solvent volume, extracting time as well as times that affect the yield of the crude polysaccharide. Also the alcohol precipitation technology was investigated by estimating the amounts and purity under the conditions of various specific gravity, concentration of alcohol and settling time. Results The optimized technological parameters were as fllows: the mixture was extracted twice by water as 10 times (w/w) for 1.5 h each time, then combined and filtered the water solutions, concentrated to equivalently 1 g (crude drugs)/mL, afterwards added the alcohol up to the concentration 80%, 4 h later, the sediment was obtained. Conclusion The parameters were suitable for the actual production condition, and the confirmed parameters would be achieved by pilot study.关键词
黄芪/丹参/三七/多糖/提取工艺/正交试验Key words
Radix astragali/ Radix salviae miltiorrhizae/ Radix notoginseng/ polysaccharide/ extracting technology/ orthogonal experiment分类
医药卫生引用本文复制引用
张永太,修彦凤,陶建生,施建国,吴浩萍..黄芪、 参、三七中多糖提取工艺考察[J].中国中医药信息杂志,2011,18(11):65-66,100,3.基金项目
基上海市科委中药现代化专项基金(06DZ19723) （06DZ19723）
上海市教委重点学科(J50302) （J50302）
上海市教委科研预算内项目(09JW10) （09JW10）
