山东医药2011，Vol.51Issue(44)：10-13,4.
新型PH-20疫苗不同注射方式对雌性豚鼠免疫应答和避孕效果的影响
Immune response and effect of contraception in female guinea pigs injected with PH-20 coupling body
摘要
Abstract
Objective To observe the immune response and contraceptive effect of guinea pigs injected with the PH-20 coupling body by different method of injection. Methods Sixty female guinea pigs were divided randomly into two groups (rectal injection group and intraperitoneal injection group) , then each group was subdivided into three groups (experimental group 1 , experimental group 2 and control group, 10 female guinea pigs in each group). To observe morphology of the coupling by electron microscopy, to test vaginal secretory immunoglobulin A (sIgA) level by double sandwich ELISA and blood sIgA by radioimmunoassay. PH-20 specific antibody level in blood was detected by competitive ELISA. Results Blood and vaginal secretions slgA and PH-20 antibody levels in rectal injection group and intraperitoneal injection group were both higher than those in control group ( P <0. 05). Blood and vaginal secretions of guinea pig sIgA and PH-20 antibody levels in experimental group 1 (add Freund complete adjuvant) were higher than those in experimental group 2 ( without adding Freund complete adjuvant) (P < 0. 05). The total positive pregnancy rate in rectal injection group was significantly different from intraperitoneal injection group ( P < 0. 05 ). Conclusions The PH-20 coupling body has a high immune activity and can induce significant humoral response, and the rectal injection is superior to the intraperitoneal injection. Freund' s complete adjuvant is able to enhance the effectiveness of two contraceptive injection.关键词
精子表面特异性透明质酸酶/免疫球蛋白A/弗氏完全佐剂/免疫刺激复合物Key words
PH-20/immunoglobulin A/complete Freund adjuvant/immunostimulating complex分类
医药卫生引用本文复制引用
金呈强,刘仿,郑碧英,董海新,周建伟,张健..新型PH-20疫苗不同注射方式对雌性豚鼠免疫应答和避孕效果的影响[J].山东医药,2011,51(44):10-13,4.基金项目
广东省卫生厅项目(A2009472) （A2009472）
山东省自然科学基金资助项目(ZR2010HL038) （ZR2010HL038）
山东省人口和计划生育委员会科学技术研究项目(2011年第19号). （2011年第19号）
