解放军医学杂志2011，Vol.36Issue(11)：1154-1158,5.
盐酸戊乙奎醚联合地塞米松对兔爆震伤急性呼吸窘迫综合征的治疗作用
Therapeutic effect of penehyclidine hydrochloride combined with dexamethasone on acute respiratory distress syndrome induced by blast injury in rabbits
摘要
Abstract
Objective To discuss the therapeutic effects of penehyclidine hydrochloride combined with dexamethasone on Acute Respiratory Distress Syndrome (ARDS) after a thoracic blast injury in rabbits. Methods Healthy New Zealand white rabbits were used to establish the ARDS model induced by a thoracic blast injury. The rabbits conforming to the diagnostic standard of ARDS were divided into 5 groups randomly: Bgroup, ARDS caused by blast ( n= 10) ; Cgroup, PHC (n=9) with low dose (0.8mg/kg); Dgroup, PHC (n=9) with higher dosed. 5mg/kg); E group, DXM ( n=8)(3mg/kg); F group, PHC+DMX (n=7) with higher dose (3mg/kg). Another set of six healthy New Zealand white rabbits were used as the control group (A group). Rabbits in the C-F group were injected with a drug at 0. 5, 12, 24, 36, and 48 h after being modeled successfully. At the same time, the rabbits in groups A and B were injected with saline in equal volumes as that in the therapeutic groups. The breathing, heart rate, and oxygenation indices, as well as the levels of TNF-α and IL-6 in the peripheral blood were monitored before the rabbits were injured, and at 6, 24, 48, and 72 h after medical treatments (initially given drugs). The rabbits were sacrificed at 72 h after they were initially given drugs. Bronchoalveolar lavage fluid (BALF) was collected in which the levels of TNF-α and IL-6 were detected by ELISA. Lung tissue specimens were taken to measure the lung water content-dry weight (W/D) ratio, and hematoxylin and eosin staining was used for observing the pathological changes of the lung tissues. Results Heartbeats, breathings, W/D ratios, and contents of TNF-a, IL-6 in peripheral blood or in BALF in group B were higher than that in group A (P<0. 05). The oxygenation index in group B was apparently lower than that in group A(P<0. 01). All indexes increased in varying degrees after the treatments with DXM and/or PHC. Group F showed better results than groups C, D, and E (P<0. 05). Conclusions PHC combined with DXM has a certain therapeutic effect on ARDS induced by a blast injury in rabbits, which is superior to any of the effects of PHC or DXM.关键词
爆震伤/呼吸窘迫综合征,成人/盐酸戊乙奎醚/地塞米松/细胞因子类/模型,动物Key words
blast injuries/ respiratory distress syndrome, adult/ penehyclidine hydrochloride/ dexamethasone/ cytokines/ models, animal分类
医药卫生引用本文复制引用
范崇熙,程庆书,汪健,朱以芳,李桂珍,张志培,李小飞..盐酸戊乙奎醚联合地塞米松对兔爆震伤急性呼吸窘迫综合征的治疗作用[J].解放军医学杂志,2011,36(11):1154-1158,5.基金项目
全军医药卫生科研基金(08G103) （08G103）
