同济大学学报（医学版）2011，Vol.32Issue(5)：23-26,51,5.DOI:10.3969/j.issn1008-0392.2011.05.006
Twist shRNA对人宫颈癌细胞体外黏附、铺展及迁移能力的影响
Effect of Twist short hairpin RNA on adhesion, spreading and migration of human cervical cancer HeLa cells in vitro
周奇 1李婷 1王国增 1赵婧 1刘岚 1吴乾渝1
作者信息
- 1. 同济大学附属东方医院妇产科,上海200120
- 折叠
摘要
Abstract
Objective To investigate the effect of shRNA of Twist gene on adhesion, spreading and migration of human cervical cancer HeLa cells. Methods Two kinds of shRNA vectors targeting to Twist gene were constructed and stably transfected into HeLa cells. The expressions of Twist gene and protein were detected by fluorescent quantitative PCR and Western blotting. The cell adhesion assay, cell spreading assay and cell migration assay were performed. Results The recombinant plasmids were successfully constructed and stably transfected into HeLa cells. The expression of Twist gene in HeLa cells was depressed significantly. The cell adhesion, spreading and migration activity were inhibited (P < 0.05). Conclusion The shRNA expressing plasmids targeting to Twist gene can inhibit the adhesion, spreading and migration activity of HeLa cells in vitro.关键词
宫颈肿瘤/Twist/RNA干扰/细胞黏附/细胞铺展/细胞迁移Key words
cervical cancer/ Twist/ RNA interference/ cell adhesion/ cell spreading/ cell migration分类
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周奇,李婷,王国增,赵婧,刘岚,吴乾渝..Twist shRNA对人宫颈癌细胞体外黏附、铺展及迁移能力的影响[J].同济大学学报（医学版）,2011,32(5):23-26,51,5.
