解放军医学杂志2011，Vol.36Issue(11)：1175-1177,3.
10例子宫乳头状浆液性癌p130蛋白及mRNA表达分析
Analysis of p130 protein and mRNA expression in ten patients with uterine papillary serous carcinoma
摘要
Abstract
Objective To examine pl30 protein and mRNA expression in uterine papillary serous carcinoma (UPSC) and their clinical and pathologic significance. Methods A total of 10 UPSC patients (Stage I) were included, with 10 cases of high-level endometrial carcinoma of the same stage taken as the control group and 10 cases of normal proliferative stage endometrium (EM) taken as the disease control group. The level of p130 protein expression was determined by hematoxylin and eosin staining, microscopic observation, and immunohistochemistry, whereas the p130 mRNA levels were examined through real-time quantitative reverse transcriptase polymerase chain reaction. The clinicopathologic analysis was carried out in combination with clinical data. Results The pl30 protein and pl30 mRNA . Expression levels in the UPSC group (0. 46+0. 01 and 0. 56+0. 06, respectively) were apparently less than that of the normal proliferative stage endometrium group (0.91 ±0.04 and 2. 81 ±0.40, respectively; P<0. 01) and also less than those in high-level endometrial carcinoma (P<0. 05). Clinicopathologic analysis shows that all patients are post-menopausal women with symptoms of irregular vaginal bleeding and the average tumor size was 7.5cm (range: 1. 2-14. 8cm). The pathologic features are same as that of high-level ovarian papillary serous carcinoma.Conclusion Reduced pl30 protein and pl30 mRNA expression in UPSC might correlate with poor prognosis in UPSC patients.关键词
子宫内膜肿瘤/视网膜母细胞瘤样蛋白质p130/免疫组织化学/逆转录聚合酶链反应Key words
endometrial neoplasms/ retinoblastoma-like protein p130/ immunochemistry/ reverse transcriptase polymerase chain reaction分类
医药卫生引用本文复制引用
丁丽娟,任兴昌,薛德彬,刘悦,夏爱丽,陈东,夏华萍,滕晓东,徐少婷,章锁江..10例子宫乳头状浆液性癌p130蛋白及mRNA表达分析[J].解放军医学杂志,2011,36(11):1175-1177,3.基金项目
宁波市科技局社会发展攻关项目(2006C100058) （2006C100058）
