同济大学学报（医学版）2011，Vol.32Issue(5)：46-51,6.DOI:10.3969/j.issn1008-0392.2011.05.010
抵抗素对人子宫内膜癌细胞增殖、黏附及侵袭的影响
Effects of resistin on proliferation, adhesion and invasion of endometrial carcinoma cells
李婷 1周奇 1王国增 1辛卫娟 1赵鑫 1吴乾渝1
作者信息
- 1. 同济大学附属东方医院妇产科,上海200120
- 折叠
摘要
Abstract
Objective To investigate the effect s of resistin on cell growth, adhesion and invasion of human endometrial carcinoma Ishikawa cells. Methods MTT assay was used to assess the proliferation and adhesion effects of resistin; and Real-Time PCR was adopted to detect the expressions of MMP-2, MMP-9, TIMP-1 and TIMP-2. Results Resistin at concentrations of 10, 50, 100 and 150 ng/ml promoted the proliferation of endometrial carcinoma cells in a time and dose-dependent manner( P < 0. 01); the effect of resistin at 100 ng/ml for 48 h was most prominent. Resistin at a range of 10-150ng/ml enhanced the adhesive ability of Ishkawa cells, with the adherence rates of 67% , 69% , 75% and 98% , respectively(P <0. 01). Resistin enhanced the expressions of MMP-2, MMP-9, and induced the unbalance of MMPs/TIMPs ratio. Conclusion Resistin can promote the proliferation of endometrial carcinoma Ishikawa cells, and enhance its adhesive and invasive ability.关键词
抵抗素/子宫内膜肿瘤/增殖/黏附/侵袭Key words
resistin/ endometrial carcinoma/ proliferation/ adhesion/ invasion分类
医药卫生引用本文复制引用
李婷,周奇,王国增,辛卫娟,赵鑫,吴乾渝..抵抗素对人子宫内膜癌细胞增殖、黏附及侵袭的影响[J].同济大学学报（医学版）,2011,32(5):46-51,6.
