广东药学院学报2011，Vol.27Issue(5)：518-520,3.DOI:10.3969/j.issn.1006-8783.2011.05.020
粘质沙雷氏菌和红曲霉跨界原生质体融合子的分子鉴定
Molecular identification of the protoplast fusant of Serratia marcescens and Monascus
摘要
Abstract
Objective To identify the inter-kingdom fusants of Senatia marcescens and Monascus with molecular biology method. Methods The random amplified polymorphic DNA ( RAPD) and functional gene amplification assay were employed to identify the nine fusants. Results Distinct DNA fragments were obtained with the screened six random primers from both parent strains and nine fusants. Except the legs similarity between the No. 5 fusant and Serratia marcescens, the similarity between the eight fusants and two parent strains was greater than that between the two parent strains. The functional mkH gene of Monascus can be amplified from the nine fusants and Monascus rubber, but not detected from the DNA of Serratia marcescens. Conclusion The nine pigmented fusants were successfully identified to be the inter-kingdom fusants from Serratia marcescens and Monascus.关键词
粘质沙雷氏菌/红曲霉/原生质体融合/随机扩增多态性/分子鉴定Key words
Serratia marcescens/ Monascus/ protoplast fusion/ RAPD/ molecular identification分类
医药卫生引用本文复制引用
周林,朱爽,杜嘉妮..粘质沙雷氏菌和红曲霉跨界原生质体融合子的分子鉴定[J].广东药学院学报,2011,27(5):518-520,3.基金项目
广东药学院博士启动基金(2006SMK01) （2006SMK01）
