中国人兽共患病学报2011，Vol.27Issue(11)：987-990,4.
表达期特异性双荧光蛋白的转基因弓形虫的构建
Construction of transgenic Toxoplasma gondii expressing dual-fluoresecent stage-specific proteins
摘要
Abstract
The conversion from tachyzoite to bradyzoite in Toxoplasma gondii accompany the expression of stage-specific proteins and this experiment aimed at constructing the dual-fluorescent stage-specific parasite. In this experiment, the tachyzoite-specified SAG1 promoter promoted the EGFP (Enhanced Green Fluorescent Protein) gene while the bradyzoite-spec-ified BAG1 promoter promoted the RFP (Red Fluorescent Protein) gene in order to construct the dual-fluorescent expression vector. Then,the vector was transformed to the parasites by electroporation. The results were confirmed by pyrimethimine screening and fluorescence. The PCR assay detected the EGFP gene and RFP gene from the genome of the dual-fluorescent parasites. It was concluded that the Toxoplasma gondii expressing dual-fluoresecent stage-specific proteins were constructed in this experiment.关键词
弓形虫/双荧光/电转/筛选Key words
Toxoplasma gondii/ dual fluorescent) electroporation/ screening分类
医药卫生引用本文复制引用
张彦雷,张厚双,蔺智兵,周勇志,曹杰,周金林..表达期特异性双荧光蛋白的转基因弓形虫的构建[J].中国人兽共患病学报,2011,27(11):987-990,4.基金项目
上海自然科学基金(10ZR1437200)和中央级公益性科研院所基本科研业务费专项(2010JB01)联合资助 （10ZR1437200）
