解放军医学杂志2011，Vol.36Issue(11)：1183-1185,3.
MHC-Ⅱ+/CD14+对大鼠免疫功能监测及预后预测的价值
Value of MHC-Ⅱ+/CD14+ in monitoring immunologic runction and prognostication of sepsis in rats
摘要
Abstract
Objective To observe the changing trend of MHC-Ⅱ/CD14+ in rats that underwent CLP (Cecal Ligation and Puncture) and its relationship with mortality and examine the significance of MHC-Ⅱ+ /CD14+ in monitoring immunologic function and prognostication of septic rats. Methods Classic CLP sepsis models were established among rats. A total of 40 rats were randomly distributed into four groups, the normal control group (C), the sham operation group (S), the one cecal perforation group (P1), and the two cecal perforations group (P2). Hood samples were taken to test the levels of MHC-Ⅱ+/CD14+ by flow cytometer on days 1, 2, 3, and 4 for groups S, P1, and P2, and on day 4 for group C. The mortality was recorded every day. (2). Based on the levels of MHC>Ⅱ+ / CD14+ , the rats were divided into three groups (≥40%, 30%-40%, and <30%). The mortality for each group was recorded. The data were processed using Chiss software. The data are expressed as (x)± s and the MHC-Ⅱ+ /CD14+ levels in each group was analyzed with an F test. The mortality rate in each group and its relationship with the level of MHC-Ⅱ+ /CD14+ were analyzed using the exact probability method (P<0. 05, with significant differences). Results Compared with groups C and S, group P1 had apparently higher mortalities on day 2, day 3 and day 4 after successful modeling and P2 does so at days 1, 2, 3, and 4 ( P<0. 05). The differences between groups C and S and between groups P1 and P2 had no statistical significance (P>0. 05). Compared with groups C and S (days 1, 2, 3, and 4 after successful modeling), the CLP rats in groups PI and P2 had apparently reduced levels of MHC-Ⅱ+ /CD14+ at all time points observed (P<0. 05 and P<0. 01) , the lowest on day 2. Group P2 drops more significantly than group P1(P<0. 01), but the difference between groups C and S had no statistical difference. The group with MHC-Ⅱ+/CD14+ <30% had more mortalities than groups ≥40% ( P< 0.05) and 30%-40% (P<0. 05). The difference in mortality between groups ≥40% and 30%-40% had no statistical significance. Conclusion MHC-Ⅱ+/CDl4+ levels may be applied to monitor the immunologic function and prognostication of septic rats.关键词
脓毒症/监测,免疫学/基因,MHCⅡ类/抗原,CD14Key words
sepsis/ monitoring, immunologic/ genes, MHC class Ⅱ/ antigens, CD14分类
医药卫生引用本文复制引用
郭剑颖,邓群,郭旭升,翁志华,刘双庆,张玉红..MHC-Ⅱ+/CD14+对大鼠免疫功能监测及预后预测的价值[J].解放军医学杂志,2011,36(11):1183-1185,3.基金项目
北京市科技计划重大项目(京科技发[2002]641号) （京科技发[2002]641号）
