中国人兽共患病学报2011，Vol.27Issue(11)：1021-1023,1034,4.
抗粉尘螨主要变应原Der fⅡ单克隆抗体的制备与鉴定
Preparation and identification of monoclonal antibodies against Der f Ⅱ allergen
摘要
Abstract
In order to prepare monoclonal antibodies from mice against Der f II allergen from Dermatophagoid.es farinae and to characterize their properties, BALB/c mice were immunized with purified recombinant Der f II allergen protein, and the splenocytes of the immunized mice were fused with NS-1 myeloma cells by hybridoma technique. Indirect ELISA was used for screening the hybridoma cell lines which could secrete antibodies against Der f II. The McAbs against Der f fl allergen were purified using affinity chromatography on immobilized Protein G to identified their specificity, subtype, titers and cross-reactvity by ELISA and Western blotting. Five hybridoma cell lines secreting McAbs against Der f fl allergen were obtained, which were determined as IgG2a subtype and good ascites titers. The five McAbs against Der f II allergen all recognized recombinant Der f II allergen and native extract from Dermatophagoides farinae. Five monoclonal antibodies against Der f II allergen were prepared successfully, which will facilitate establishing detection method of Der Ⅱ allergen.关键词
Der fⅡ/粉尘螨/主要变应原/单克隆抗体/特性鉴定Key words
Der fⅡ/ Dermatophagoides farinae / major allergen/ monoclonal antibody/ characterization分类
医药卫生引用本文复制引用
刘晓宇,吉坤美,李荔,邹菊,刘志刚..抗粉尘螨主要变应原Der fⅡ单克隆抗体的制备与鉴定[J].中国人兽共患病学报,2011,27(11):1021-1023,1034,4.基金项目
国家863计划项目(No.2006AA02A231)、国家自然科学基金(No.810713788)、粤港关键领域重点突破项目(No.20054982207) （No.2006AA02A231）
