中国人兽共患病学报2011，Vol.27Issue(11)：953-957,5.
汉防己甲素对氟康唑抗白念珠菌生物膜增效活性的初步研究
Preliminary study on tetrandrine as a synergist to fluconazole against biofilms of Candida albicans
摘要
Abstract
The aim was to investigate the synergistic effect of tetrandrine (TET) on fluconazole (FLO to Candida albi-cans biofilms. The biofilms of C. albicans was built, and the minimum inhibitory concentration (MIC) of FLC alone as well as in combination with TET on each stage of biofilms forming were determined by the CLSI proposed M27-A microdilution method. The MIC of FLC alone and in combination with TET on re-suspended C. albicans biofilms were tested. In initial stage (Oh), the MIC50 of FLC alone and combined with TET to the C. albicans biofilms were 0. 25-64 g/mL and 0. 125-16 g/mL respectively (P=0. 002). In the early stage (4thh), the MIC50 were 8-256μg/mL and 1-64 μg/mL (P = 0. 000). In the middle (24lhh) and advanced stages (48th h), both the MIC50 were >1024 μg/mL. The MIC of FLC alone to the re-suspended C. albicans biofilm (1×103 CFU/mL) were 0. 25-64 μg/mL and 0. 125-16 μg/mL when combined with TET. Both the MIC of FLC alone and in combination with TET were >64 μg/mL in the cells counts of 1×10s CFU/mL. In vitro, TET acts synergistical-ly with fluconazole in the activity against the C. albicans biofilms in initial stage (Oh) and early stage (4thh) , without synergistic effect in middle stage (24lhh) and advanced stage (48th h) ; there is synergistic effect of tetrandrine to fluconazole against the re-suspended thalli of C. albicans biofilm (1×103CFU/mL) ,and no synergy against the thalli (1×106CFU/mL).关键词
汉防己甲素/氟康唑/白念珠菌/生物膜/增效作用Key words
tetrandrine/ fluconazole/ Candida albicans/ biofilm/ synergy分类
医药卫生引用本文复制引用
李水秀,刘朝红,张宏,张晓利,宋延君..汉防己甲素对氟康唑抗白念珠菌生物膜增效活性的初步研究[J].中国人兽共患病学报,2011,27(11):953-957,5.基金项目
国家自然科学基金(30972660)、广州市科技计划项目和国家大学生创新实验计划项目(10A32070407)联合资助 （30972660）
