中国人兽共患病学报2011，Vol.27Issue(11)：966-969,4.
我国细粒棘球绦虫新疆分离株AgB8/3亚基克隆表达及其检测囊型包虫病价值分析
Cloning, expression and serological evaluation of the antigen B 8/3 subunit from isolate of Echinococcus granulosus from Xinjiang, China
摘要
Abstract
In the present study, total RNA was extracted from E. granulosus protoscoleces collected from a sheep hyda-tid cyst from Xinjiang, and AgB8/3 fragments were obtained by RT-PCR using the extrcted RNA and cloned into pGEX-3X vector. The recombinant plasmid pGEX-3X- AgB8/3was transformed into Escherichia coli BL21 strains and induced by isopro-pyl-(3-D- thiogalactopyranoside (IPTG). The expressed recombinant protein was purified by affinity chromatography and was e-valuated for the diagnosis of CE by ELISA compared with rAgB8/l and rAgB8/2. The results of restriction enzymes digestion and sequencing analysis showed that plasmid pGEX~3X-AgB8/3 was successfully constructed and rAgB8/3 was successfully expressed in prokaryotic cell induced by IPTG. Detection on 118 serum samples of patients with cyst echinococcosis showed a sensitivity of 55. 9% (66/118) , while 15 positives out of 34 alveolar echinococcosis, 3 positives out of 59 patients with other parasitic disease and none of 60 healthy persons with a specificity of 87. 4% (125/143). There was no statistical difference in the sensitivity between rAgB8/3 and rAgB8/l(P>0. 05), but sensitivity of rAgB8/2 was higher than that of rAgB8/3(P<0. 05). There was no statistical difference in the specificity among the three recombinant subunits (P>0. 05).关键词
细粒棘球蚴/AgB8/3亚基/基因克隆和表达/ELISAKey words
Echinococcus granulosus, AgB8/3 subunits cloning and expression/ ELISA分类
医药卫生引用本文复制引用
高春花,崔刚,汪俊云,杨明涛,石锋,朱慧慧..我国细粒棘球绦虫新疆分离株AgB8/3亚基克隆表达及其检测囊型包虫病价值分析[J].中国人兽共患病学报,2011,27(11):966-969,4.基金项目
国家科技重大专项(No.2008ZX10004 011,2009ZX10603和2009ZX10004-201)和科技支疆课题(No.200891130)联合资助 （No.2008ZX10004 011,2009ZX10603和2009ZX10004-201）
