中国人兽共患病学报2011，Vol.27Issue(11)：975-979,5.
脂多糖对约氏疟原虫感染DBA/2小鼠感染早期的免疫调节作用
Regulatory effect on lipopolysaccharide for lethal Plasmodium yoelii infection in early stage
摘要
Abstract
This paper discusses the immune regulatory mechanism of the TLR4 agonist-lipopo in 6 to 8 weeks lysaccha-ride (LPS) in the lethal form of Plasmodium yoelii infection in early stage. Female DBA/2 mice were randomly divided into experimental and control groups, intraperitoneally inoculating 1×106 Plasmodium yoelii 17×L(Pyl7×L) parasitized red blood cells. The experimental group was given LPS (25μg/once) intravenously after infection day 2. Parasitemia were dynamicly observed during the whole infection course. In infected day 0, day 3, and day 5, the number of changes of dendritic cell subsets, dendritic cell surface TLR4, and activation on the effect of T cells in the spleen were detected by flow cytometry (FACS). Results demonstrated that the level of parasitemia in LPS treatment mice after infection day 5 to day 8 was significantly lower than that in the control group; on day 3 and day 5 post infection, compared with the control group, the number of CDllc+CDllb+ DCs and CDllc+CD45R/B220"1" DCs subsets in the spleen in LPS treated mouse were significantly increased; on day 3,CDllc+ TLR4+ DCs in the spleen were significantly increased; on day 5, CD4+CD69f T lymphocytes in the spleen were significantly increased. In conclusion, through the activation of TLR4 in early stage of P. yl7 X L infection, LPS could promote the DC maturation, change its subpopulation proliferation model, and further strengthen the establishment of Thl-type immune response. Then it obviously decreases the parasitemia. Therefore it would provide new targets for effective antimalarial vaccine development.关键词
脂多糖/致死型约氏疟原虫/免疫调节Key words
lipopolysaccharide/ Plasmodium yoelii 17XL/ immunoregulation分类
医药卫生引用本文复制引用
李莹,刘军,潘艳艳,王各各,延娟,郑丽,冯辉,曹雅明..脂多糖对约氏疟原虫感染DBA/2小鼠感染早期的免疫调节作用[J].中国人兽共患病学报,2011,27(11):975-979,5.基金项目
国家自然科学基金项目(No.30800961)资助 （No.30800961）
