动物学研究2011，Vol.32Issue(5)：465-475,11.DOI:10.3724/SP.J.1141.2011.05465
斐豹蛱蝶线粒体基因组全序列的测定和分析
Complete mitochondrial genome of the laced fritillary Argyreus hyperbius (Lepidoptera: Nymphalidae)
摘要
Abstract
We investigated the complete mitochondrial genome (mitogenome) of A rgyreus hyperbius.The 15156 bp long genome harbored the gene content (13 protein coding genes,22 tRNA genes,2 rRNA genes and an A+T-rich region) and the gene arrangement was identical to all known lepidopteran mitogenomes.Mitogenome sequence nucleotide organization and codon usage analyses showed that the genome had a strong A+T bias,accounting for A+T content of 80.8%,with a small negative AT skew (-0.019).Eleven intergenic spacers totaling 96 bp,and t4 overlapping regions totaling 34 bp were scattered throughout the whole genome.As has been observed in other lepidopteran species,12 of the 13 protein-coding genes (PCGs) were initiated by ATN codons,while the COI gene was tentatively designated by the CGA codon.A total of 11 PCGs harbored the complete termination codon TAA,while the COⅠ and COⅡ genes ended at a single T residue.All of the 22 tRNA genes showed typical clover structures except that the tRNAser(AGN) lacks the dihydrouridine (DHU) stem which is replaced by a simple loop.The intergenic spacer sequence between the tRNAser(AGN) and ND 1 also contained the ATACTAA motif,which is conserved in all other lepidopterans as well.Additionally,the 349 bp A+T-rich region was not comprised of large tandem repetitive sequences,but harbored a few structures common to other lepidopteran insects,such as the motif ATAGA followed by a 20 bp poly-T stretch,a microsatellite-like (AT)9 element preceded by the ATTTA motif,and a 5 bp poly-A site present immediately upstream of tRNAMet.The mitochondrial genomic sequence features found in this study not only contribute to genetic diversity information of the group,but also are useful in future studies of the endangered nymphalid butterfly in population genetic dynamics,species conservation,phylogeography and evolution.关键词
斐豹蛱蝶/蛱蝶科/鳞翅目/线粒体基因组Key words
Argyreus hyperbius/Nymphalidae/Lepidoptera/Mitochondrial genome分类
生物科学引用本文复制引用
王晓灿,孙晓燕,孙倩倩,张大秀,胡静,杨群,郝家胜..斐豹蛱蝶线粒体基因组全序列的测定和分析[J].动物学研究,2011,32(5):465-475,11.基金项目
安徽省高校省级自然科学研究重点项目(KJ 2010A 142) （KJ 2010A 142）
中国科学院知识创新工程重要方向项目(KZCX22YW2JC 104) （KZCX22YW2JC 104）
现代古生物学和地层学国家重点实验室开放基金(104143)This work was supported by the Provincial Key Project of the Natural Science Foundation from Anhui Province.China (KJ2010A142).the Chinese Academy of Sciences (KZCX22YW2JC104).the CAS/SAFEA International Pmrnership Program for Creative Research Teams,and the State Key Laboratory of Palaeobiology and Stratigraphy,Nanjing Institute of Geology and Palaeontology,Chinese Academy of Sciences (104143) （104143）
