广西植物2011，Vol.31Issue(5)：584-587,583,5.DOI:10.3969/j.issn.1000-3142.2011.05.006
应用RAPD和ISSR标记对24份花生栽培种材料进行遗传多样性分析
Genetic diversity in 24 peanut cultivars as revealed by RAPD/ISSR profiling
摘要
Abstract
Genetic diversity in 24 accessions of cultivated peanut materials from China was evaluated based on RAPD/ ISSR profiling. Of the RAPD primers and ISSR primers tested,13 and 10 primers produced a total of 123 and 87 re-peatable, clear and readable bands,among which 47. 15% and 57. 47% were polymorphic,respectively. On average,a primer resulted in 9. 5 and 8. 7 bands,of which 4. 7 and 5. 7 were polymorphic. A dendrogram was constructed using UPGMA algorithm based on Nei and Li's similarity coefficient,which divided the 24 peanut materials into 4 groups. Silihong,Sanyou 523,Jihua 2,Huayu 16 and Yueyou 7 were in one group. Huyu 20 and Zhonghua 8 clustered together. Heihuasheng formed a separated group,and the rest peanut materials tested fell in a group. RAPD and ISSR are useful for the genetic diversity studies of the cultivated peanut,and have potential in characterization of peanut germ-plasm and gene mapping.关键词
花生/RAPD/ISSR/聚类分析/遗传多样性Key words
peanut/ RAPD/ ISSR/ cluster analysis/ genetic diversity分类
农业科技引用本文复制引用
闫苗苗,魏光成,谭秀华,王传堂..应用RAPD和ISSR标记对24份花生栽培种材料进行遗传多样性分析[J].广西植物,2011,31(5):584-587,583,5.基金项目
山东省自然科学基金(Y2008D11)[Supported by the Natural Science Foundation of Shandong Province(Y2008D11)] （Y2008D11）
