南方农业学报2011，Vol.42Issue(9)：1144-1147,4.
PCR-DGGE技术在水牛瘤胃产甲烷菌多样性分析中的应用
Diversity analysis of rumen methanogens in buffalos using PCR-DGGE technique
摘要
Abstract
The present experiment was conducted to analyze the diversity of rumen methanogens in buffalos. [ Method ]The rumen contents were collected from sixteen local swamp buffalo in Guangxi to extract the total DNA, and the 16S rKNA gene sequences were amplified and analyzed using universal primers and DGGE technique. The obtained 7 dominant bands in DGGE gel were cloned and sequenced. [Result]After cloning, nine sequences were obtained, of which 5 were archaea and 4 were not. Three sequences amongst 5 archaea showed 99% homology with Methanobrevibacter, and one sequence showed 98% of homology with Methanobrevibacter. The other sequence unclassified in Euryarchaeota showed 98% homology with Thermoplasmatales. In addition, four non archaea sequences were belonged to Bacteroidetes Firmieutes and Proteobacteria, but their homologies were relative less. [ Conclusion ] DGGE technique was found useful in analyzing the diversity of rumen methanogens in buffalos.关键词
水牛/瘤胃/产甲烷菌/PCR-DGGE/多样性分析Key words
buffalo/ rumen/ methanogens/ PCR-DGGE/ diversity analysis分类
农业科技引用本文复制引用
刘园园,姜伟伟,秦红玉,张卉,王士长..PCR-DGGE技术在水牛瘤胃产甲烷菌多样性分析中的应用[J].南方农业学报,2011,42(9):1144-1147,4.基金项目
广西大学研究生创新计划项目(2007105930905M079) （2007105930905M079）
