南方农业学报2011,Vol.42Issue(9):1148-1150,3.
广西中华草龟遗传多样性的RAPD分析
Analysis of genetic diversity amongst Chinemys reevesii in Guangxi using RAPD markers
摘要
Abstract
The current experiment was conducted to analyze the genetic diversity amongst Chinemys reevesii accessions of Guangxi in order to protect and characterize the germplasm resources and promote its suitable development and selection breeding. [Method]Genetic diversity of Chinemys reevesii accessions of Guangxi was analyzed using RAPD markers. [ Result ]The results showed amplification of total of 267 bands from genomic DNA of 7 Chinemys reevesii accessions with an average of 38 bands per accession. Each random primer produced over 15 bands on an average. The size of fragments ranged from 100-2000 bp. Out of 267 bands, 177 were polymorphic with 66.29% polymorphism frequency. The genetic distance between individuals was 0.1360-0.3609, the average genetic distance was 0.2092±0.0623. [Conclusion] Abundant genetic diversity was observed amongst different accessions of Chinemys reevesii in Guangxi.关键词
中华草龟/遗传多样性/RAPD分析/广西Key words
Chinemys reevesii/ genetic diversity/ RAPD/ Guangxi分类
生物科学引用本文复制引用
朱学峰..广西中华草龟遗传多样性的RAPD分析[J].南方农业学报,2011,42(9):1148-1150,3.基金项目
广西科技攻关项目(桂科攻0428011) (桂科攻0428011)