植物学报2011，Vol.46Issue(6)：625-631,7.DOI:10.3724/SP.J.1259.2011.00625
水稻短根毛突变体Ossrh2的表型分析与基因定位
Phenotypic Analysis and Gene Mapping of a Short Root Hair Mutant Ossrh2 in Rice (Oryza sativa)
摘要
Abstract
A rice (Oryza sativa) mutant with short root hairs (0ssrh2) was isolated from a T-DNA insertion mutant library of rice in the Zhonghua11 background. Except for root hairs, Ossrh2 did not differ from the wild type. The number and length of root hairs of Ossrti2 were only 61.4% and 22.8%, respectively, that of the wild type, and hairs were thicker. The root hairs of Ossrh2 were also morphologically abnormal and showed distortion, swelling and branching. Genetic analysis revealed that the mutant phenotype was controlled by a single recessive nuclear gene. PCR analysis of T2 and F2 mutants showed that the phenotype was not caused by T-DNA insertion. To map the OsSRH2 gene, we generated an F2 population by crossing the mutant 0ssrh2 with the Kasalath wild type. 0sSRH2 was first mapped between the microsatellite markers RM6370 and RM474 on the short arm of chromosome 10 with genetic distance of 1.1 and 3.0 cM, respectively. Then, 3 new polymorphic sequence-tagged site markers were developed in the region. OsSRH2 was finally mapped between markers S1227 and S1531 with a physical distance of 304 kb, which would help in its cloning.关键词
基因定位/遗传分析/水稻/短根毛突变体Key words
gene mapping/ genetic analysis/ Oryza sativa/ short root hair mutant引用本文复制引用
丁沃娜,童艳丽,宁永强,朱世华..水稻短根毛突变体Ossrh2的表型分析与基因定位[J].植物学报,2011,46(6):625-631,7.基金项目
国家自然科学基金(No.31071388)和宁波市自然科学基金(No.2011A610001) （No.31071388）
