南方农业学报2011，Vol.42Issue(9)：1029-1034,6.
玉米种质材料遗传多样性的ISSR分析
Genetic diversity of maize (Zea mays L.) accessions using inter-simple sequence repeat (ISSR) markers
VuVan Liet 1Nguyen Thi Thuy Linh 2Nguyen Thi Thuy 2VuThi Bich Hanh 1PhamQuangTuan 1Nguyen Thi Phuong Thao2
作者信息
- 1. 河内农业大学水稻研究所,越南河内
- 2. 河内农业大学生物技术系,越南河内
- 折叠
摘要
Abstract
The present study was conducted to analyze the characterization of the genetic diversity among local maize accessions in the North mountain region of Vietnam using ISSR markers.[ Method ]The ISSR technique and 10 primers were used to study genetic diversity among 21 maize accessions consisting of 12 normal and 9 waxy maize accessions,collected from 3 provinces in the North mountain region of Vietnam and Laos.[ Result]A total of 108 ISSR fragments were detected and all of them were polymorphic (100%).Polymorphism information content (PIC) values of ISSR primers ranged from 0.10-0.39.The average PIC value for each primer was 0.24.The resolving power (Rp) value ranged from 14.29-0.48 with an average of 4.48 per primer.All of tested ISSR primers with the exception of ISSR-T1 generated unique fragments (present only in one accession) in 13 among 21 maize accessions.Based on UPGMA analysis,using 70% genetic similarity as the cutoff,a dendrogram was constructed and 21 maize accessions were grouped into three clusters.The similarity coefficients among accessions ranged from 0.52-0.90.[Conclusion ]ISSR markers provided information on genetic diversity of maize accessions which was useful for collection,conservation and breeding programs in Vietnam.关键词
ISSR分子标记/遗传多样性/玉米地方品种/聚类分析/PCRKey words
ISSR markers/ genetic diversity/ maize landraces/ UPGMA analysis/ PCR分类
农业科技引用本文复制引用
VuVan Liet,Nguyen Thi Thuy Linh,Nguyen Thi Thuy,VuThi Bich Hanh,PhamQuangTuan,Nguyen Thi Phuong Thao..玉米种质材料遗传多样性的ISSR分析[J].南方农业学报,2011,42(9):1029-1034,6.
