应用生态学报2011，Vol.22Issue(11)：3039-3046,8.
重要昆虫病原真菌粉棒束孢种群的遗传分化
Genetic differentiation of Isaria farinosa populations in Anhui Province of East China
摘要
Abstract
Isaria farinosa is an important entomopathogenic fungus. By using ISSR, this paper studied the genetic heterogeneity of six /. Farinosa populations at different localities of Anhui Province, East China. A total of 98.5% polymorphic loci were amplified with ten polymorphic primers, but the polymorphism at population level varied greatly, within the range of 59.6% -93.2%. The genetic differentiation index ( Gst) between the populations based on Nei' s genetic heterogenesis analysis was 0.3365, and the gene flow ( Nm) was 0.4931. The genetic differentiation between the populations was lower than that within the populations, suggesting that the genetic variation of /. Farinosa mainly come from the interior of the populations. The UPGMA clustering based on the genetic similarities between the isolates revealed that the Xishan population was monophylectic, while the other five populations were polyphylectic, with the Yaoluoping population being the most hetero-genic and the Langyashan population being the least heterogenic. No correlations were observed between the geographic distance and the genetic distance of the populations. According to the UPGMA clustering based on the genetic distance between the populations, the six populations were classified into three groups, and this classification was accorded with the clustering based on geographic environment , suggesting the effects of environmental heterogeneity on the population heterogeneity.关键词
粉棒束孢/种群结构/遗传多样性/种群异质性/ISSRKey words
Isaria farinosa/ population structure/ genetic diversity/ population heterogeneity/ISSR.分类
农业科技引用本文复制引用
孙召红,栾丰刚,张大敏,陈名君,王滨,李增智..重要昆虫病原真菌粉棒束孢种群的遗传分化[J].应用生态学报,2011,22(11):3039-3046,8.基金项目
国家自然科学基金项目(30972368)和科技部基础性工作专项(2008FY210500)资助. （30972368）
