应用生态学报2011，Vol.22Issue(11)：3047-3052,6.
引进天敌莲草直胸跳甲的遗传多样性
Genetic diversity of introduced natural enemy Agasicles hygrophila
摘要
Abstract
Flea beetle Agasicles hygrophila Selman & Vogt, the most effective natural enemy of alligator weed Alternanthera philoxeroideis, is originated from South America, and has been introduced into China for more than 20 years to control the weed. In this study, the technique of random amplification of polymorphic DNA ( RAPD) was applied to assess the genetic diversity of four A. Hygrophila geographic populations in Guangzhou, Chongqing, Kunming, and Fuzhou of China, and, taking the population in Florida of US as the reference, the relationships between the genetic differentiation and geographic differentiation among and within the populations were explored at molecular level. Thirteen primers that produced clear and reproducible products were selected from 111 random primers to amplify the 25 samples of the five A. Hygrophila populations. The total polymorphic rate was 42. 0% , and the polymorphic rate of Florida population was significantly higher than that of four China populations. The genetic differentiation among the four China populations was 25.5%. The cluster analysis by UPGMA indicated that there was a positive correlation between the genetic distance and geographic distance of different A. Hygrophila geographic populations.关键词
莲草直胸跳甲/引种/地理种群/遗传多样性/RAPDKey words
Agasicles hygrophila/introduction/geographic population/genetic diversity/RAPD.分类
生物科学引用本文复制引用
韩政,赵龙龙,马瑞燕,郝炯,贾栋..引进天敌莲草直胸跳甲的遗传多样性[J].应用生态学报,2011,22(11):3047-3052,6.基金项目
教育部新世纪优秀人才计划项目(NCET-08-0886)、国家自然科学基金项目(31070480)和山西省归国留学人员科研项目(2009036)资助. （NCET-08-0886）
