武警医学2011，Vol.22Issue(10)：849-851,855,4.
HSP70在小鼠睾丸巨细胞病毒感染中的表达及作用
Expression and function of HSP70 in MCMV infected male mice
熊锦文 1赵玉梅 1徐红 1于建萍 1胡廉 2官黄涛 2熊承良2
作者信息
- 1. 430061 武汉,武警湖北总队医院妇产科
- 2. 430030 武汉,华中科技大学同济医学院计划生育研究所生殖医学中心
- 折叠
摘要
Abstract
Objective To study the expression of HSP70 in different periods of MCMV infection in mouse testes and discuss possible functions of HSP70 in the pathogenic mechanism of MCMV and organisms resistance to MCMV infection. Methods Following the establishment of a murine testicular MCMV infection model, experimental and control groups were set up. In different periods of MCMV infection and using immunohistochemical SP method, the expression levels and location of HSF70 in testicular tissue were detected to explore its relationship with MCMV expression and testicular tissue lesions. Results After mouse testes had been inoculated with virus, the level of HSP70 expression varied with the period of infection. It began to increase from Dl PI (P <0.01) and reached maximum in D6 ~ D9 PI( P <0.01). At this time MCMV achieved its greatest proliferation while testis underwent the most serious pathological inflammatory changes. As infection continued, the expression of HSP70 was reduced, virus multiplication slowed down, and the lesion level of testis decreased. Conclusions The expression of MCMV - infected HSP70 in testis is related to MCMV multiplication and the level of testicular lesions. HSP70 possibly contributes to the pathogenic mechanism of MCMV and organisms' resistance to MCMV infection.关键词
鼠巨细胞病毒/睾丸/热休克蛋白70Key words
murine cytomegalovirus( MCMV) / testis/ heat shock protein 70分类
医药卫生引用本文复制引用
熊锦文,赵玉梅,徐红,于建萍,胡廉,官黄涛,熊承良..HSP70在小鼠睾丸巨细胞病毒感染中的表达及作用[J].武警医学,2011,22(10):849-851,855,4.
