湖北医药学院学报2011，Vol.30Issue(5)：480-482,3.
人视网膜Müller细胞的培养
Culture of Human Retinal Müller Cells with Enzymatic Digestion Method
蔡维 1程扬 2钱航 3陈龙 3赵黎炳3
作者信息
- 1. 湖北医药学院附属东风医院眼科,湖北十堰442008
- 2. 华中科技大学同济医学院附属协和医院眼科,湖北武汉430022
- 3. 湖北医药学院附属东风医院实验中心,湖北十堰442008
- 折叠
摘要
Abstract
Objective To establish a simple method for the culture of human Muller cells. Methods The human retinal Muller cells were cultured by single enzymatic digestion method and were identified by transmission electron microscope a-nalysis and immunofluorescence staining. Results Human retinal Muller cells showed abundant cytoplasraic,mitochondrion, rough endoplasmic ieticulum,glycogen particles and characteristic 8-10 ran intracellular filaments in the cytoplasm under the transmission electron microscope. More than 95% cells showed green fluorescene in the cytoplasm under fluorescene microscope. Conclusion Human retinal Muller cells could be cultured by a simple enzymatic digestion method successfully.关键词
Müller细胞/细胞培养/视网膜Key words
Muller cell/Cell culture/Retinal分类
医药卫生引用本文复制引用
蔡维,程扬,钱航,陈龙,赵黎炳..人视网膜Müller细胞的培养[J].湖北医药学院学报,2011,30(5):480-482,3.
