西安交通大学学报（医学版）2011，Vol.32Issue(6)：664-667,4.
β-榄香烯增强柔红霉素杀伤人类白血病细胞的作用及机制
Mechanism of synergistic anti-tumor effect of β-elemene with daunorubicin on human leukemia cells
摘要
Abstract
Objective To explore the reversing effect of P-elemene on the multi-drug resistance of leukemia cells and the expressions of poly-ADP-ribose polymerase protein (PARP) and P-glycoprotein (P-gp) in K562/DNR cells. Methods Trypanblue dye exclusion method was used to detect the sensitivity of K562/DNR cells to p-elemene and the reversing effect of P-elemene on multi-drug resistance. Cell apoptosis was determined by flow cytometry after propidium iodide staining. Intracellular accumulation of daunorubicin in K562/DNR cells was observed by fluorescent-spectrophotometry. Expressions of the proteins were detected by Western blot. All experiment data were dealt with by SPSS (13.0 software package). Results The inhibitory effect of P-elemene on both K562 and K562/DNR was detected in a dose-dependent manner. Low-cytotoxic dose of P-elemene could obviously decrease the IC50 value of K562/DNR cells to daunorubicin (IC50=0.68±0.43, P＜0.05). The reversing fold was 1. 91. Low-cytotoxic dose of P-elemene could evidently increase the intracellular accumulation of daunorubicin and remarkably induce the activated fragment of PARP and decrease P-gp expression in K562/DNR cells. Conclusion P-elemene can partially reverse the multi-drug resistance of K562/DNR cells to daunorubicin, which is related to the increased accumulation of intracellular drugs, the activated fragment of PARP and the decreased expression of P-gp.关键词
白血病/β-榄香烯/多药耐药/聚腺苷二磷酸聚合酶/柔红霉素/细胞凋亡Key words
leukemia cells P-elemene/multi-drug resistance/poly-ADP-ribose polymerase protein (PARP)/daunorubicin (DNR)/apoptosis分类
医药卫生引用本文复制引用
张晔,曲秀娟,刘云鹏,杨向红,侯科佐,滕月娥,张敬东..β-榄香烯增强柔红霉素杀伤人类白血病细胞的作用及机制[J].西安交通大学学报（医学版）,2011,32(6):664-667,4.基金项目
国家自然科学基金资助项目(No.30901736) （No.30901736）
辽宁省教育厅一般项目(No.L2010641) （No.L2010641）
