西安交通大学学报（医学版）2011，Vol.32Issue(6)：687-689,727,4.
重度间歇性低氧对大鼠认知功能及海马超微结构的影响
Effects of intermittent serious hypoxia on the cognitive function and hippocampus ultra-microstructure in rats
摘要
Abstract
Objective We set up an intermittent hypoxia (IH) animal model by simulating the features of obstructive sleep apnea-hypopnea syndrome (OSAHS) to observe the effects on cognitive function and hippocampus ultra-structure in rats exposed to 50 mL/L IH. Methods Totally 48 mature male Wistar rats were randomly assigned into two groups: 50 mL/L IH (50 mL/L IH) group and unhandled control (UC) group. The compressed air was given to UC group while rats in IH groups suffered from 8-hour IH every day for 2. 4. 6 or 8 weeks, respectively. The cognitive function of rats in 50 mL/L IH and UC groups was assessed with the Morris water maze (MWM) test, and the hippocampus ultra-microstructure was also observed. Results Compared with that in UC group, the synaptic ultra-structure of nerve cell nucleus, mitochondria and rough endoplasmic reticulum was significantly injured in 50 mL/L IH group in a time-dependant manner. MWM test showed that with prolonged hypoxia, the escaping latency was prolonged [(49.17±8.87)s at week 2 and (68.42±7.91)s at week 8], all longer than those in UC group [(25.66+2.97)s at week 2 and (25.29 + 3.07)s at week 8, respectively]. The frequency of crossing the platform was decreased in 50 mL/L IH group (7. 68+ 1. 6 at week 2 and 2. 87 ± 0. 92 at week 8, respectively), all lower than those in UC group (11.57±2.18 at week 2 and 10.65± 1.26 at week 8, respectively). Conclusion Intermittent hypoxia can cause cognitive dysfunction. This damage is related to the neuronal ultra-structure injury from prolonged intermittent hypoxia.关键词
重度间歇性低氧/认知功能/水迷宫/海马超微结构/电镜/阻塞性睡眠呼吸暂停低通气综合征(OSAHS)Key words
intermittent serious hypoxia/cognitive functioni Morris water maze/neuronal ultra-structure/electron microscope/obstructive sleep apnea-hypopnea syndrome (OSAHS)分类
医药卫生引用本文复制引用
李琳,王红阳,陈宝元,赵雅宁,杨林,韩晓庆,张盼盼..重度间歇性低氧对大鼠认知功能及海马超微结构的影响[J].西安交通大学学报（医学版）,2011,32(6):687-689,727,4.基金项目
河北省科技厅资助项目(No.09276103D-11) （No.09276103D-11）
