诊断学理论与实践2011，Vol.10Issue(5)：449-453,5.
实时荧光PCR快速检测耐甲氧西林金黄色葡萄球菌的临床评估
Real-time fluorescence PCR for detection of methicillin-resistant Staphylococcus aureus
李耀华 1费春荣 2叶爱青2
作者信息
- 1. 国家食品药品监督管理局医疗器械技术审评中心,北京100044
- 2. 浙江大学医学院附属邵逸夫医院检验科,浙江杭州310016
- 折叠
摘要
Abstract
Objective Using real-time fluorescence PCR for the detection of methicillin-resistant Staphylococcus aureus (MRSA) and to assess it clinical value. Methods Real-time fluorescence PCR and isolation-culture-K-B paper disk diffusion method were performed to analyze 62 Staphylococcus aureus strains and 35 non-Staphylococcus aureus strains isolated clinically. Samples with different testing results by these two methods were further examined by sequencing method for final identification. Results Sixty-two Staphylococcus aureus were identified by culture method and real-time fluorescence PCR method. Of the 62 clinical isolated Staphylococcus aureus 41 isolates were defined as MRSA by K-B paper disk diffusion method, and 43 isolates were defined as MRSA by real-time fluorescence PCR. The two isolates defined as positive by real-time fluorescence PCR and negative by K-B paper disk diffusion method were proved to be MRSA positive by sequencing method. The sensitivity of PCR for detecting MRSA was lxlO3 cfu/mL Conclusions Realtime fluorescence PCR could be used clinically for the rapid detection of MRSA with high clinical value.关键词
耐甲氧西林金黄色葡萄球菌/细菌培养/实时荧光聚合酶链反应Key words
Methicillin-resistant Staphylococcus aureus/ Bacteria culture/ Real-time fluorescence PCR分类
医药卫生引用本文复制引用
李耀华,费春荣,叶爱青..实时荧光PCR快速检测耐甲氧西林金黄色葡萄球菌的临床评估[J].诊断学理论与实践,2011,10(5):449-453,5.
