摘要
Abstract
Objective To explore the best treatment method of breast milk for detecting HBV-M with enzyme-linked im-munosorbent assay (ELISA). Methods According to HEeAg and Pres, of blood serum, negative or positive, 144 HBV-positive parturients were divided to HBeAg-positive and PreS,-positive group (n = 16), HBeAg-positive group (n = 11) , PreS1-positive group (n =5), and HBeAg-negative and PreS1-negative group(n = 112). Colostrums were collected with asepsis from day 2 to day S after parturition. All the samples were treated with standing brood qualitation of colostrums, standing brood qualitation of inter-layer breast milk after cold preservation, and vibratory brood of colostrums respectively. HBV-M was detected by ELISA. Results In HBeAg-positive and PreS,-positive group, HBeAg-positive group and PreS,-positive group, positive rates of HBsAg treated with the 3 methods were 100.0% or close to 100.0%. Detection rate of HBsAg, HBeAg, and PreS1 with vibratory brood of colostrums were higher than those with the other 2 treatment methods, and the differences were significant (P<0.05). The detection rates of HBsAg, HBeAg, and PreS, were at the same level with the same method, but the detection rates of anti-Hbe and anti-HBc were lower than that of anti-HBc, and the differences were significant (P<0.05). Conclusion HBV in HBV-positive parturient can be detected with vibratory brood, if there is no access to HBV-DNA detection facilities.关键词
肝炎病毒,乙型/血传病原体/乳汁/酶联免疫吸附测定Key words
Hepatitis B virus/ Blood-borne pathogen/ Lac feminium/ Enzyme-linked immunosorbent assay分类
医药卫生
