西安交通大学学报（医学版）2011，Vol.32Issue(6)：754-757,4.
长期应用糖皮质激素对大鼠股骨头骨组织MMPs/TIMPs系统的影响
Effects of long-term glucocorticoid medication on MMPs/TIMPs system in bone tissues of rat femoral head
摘要
Abstract
Objective To investigate the effects of long-term glucocorticoid medication on matrix metalloproteinase (MMPs)/tissue inhibitor of matrix metalloproteinases (TIMPs) system in bone tissues of rat femoral head, and to discuss the related action mechanism of glucocorticoid-induced necrosis of femoral head. Methods Forty healthy adult SD rats were randomly divided into 4 groups: high-dose glucocorticoid group (HD), medium-dose glucocorticoid group (MD), low-dose glucocorticoid group (LD) and control group, 10 rats in each. HD, MD and LD groups were differently treated with intramuscular injection of prednisolone (25, 12. 5, and 7 mg/kg body weight) twice a week, respectively; the control group was only treated with the same volume of sodium chloride. After 4 weeks' intervention, osteonecrosis of left femoral heads was detected by HE staining; the total RNA of right femoral head bone tissues total RNA was extracted; and the expression levels of MMP-2, MMP-9, TIMP-1 and TIMP-2 mRNAs were examined by real-time quantitative polymerase chain reaction. Results The early-stage necrosis rate of femoral head was 83.33% (5/6), 66.67% (6/9), 30% (3/10) and 0 (0/10) in HD, MD, LD and control groups, respectively. MMP-2 and MMP-9 mRNA expression levels in HD, MD and LD groups were higher than those in control group and were positively correlated with the hormone dosage. HD and MD groups differed from control group significantly (P＜0.05). TIMP-1 and TIMP-2 mRNA expression levels were significantly lower in HD, MD and LD groups than in control group, and were negatively correlated with the hormone dosage (P＜0.05). Conlusion Glucocorticoid-induced necrosis of femoral head may be related to glucocorticoid's regulation of the expression levels of MMPs/TIMPs mRNAs.关键词
糖皮质激素/股骨头/骨坏死/细胞因子Key words
glucocorticoid/femoral heads osteonecrosis/cytokine分类
医药卫生引用本文复制引用
王建忠,王坤正,时志斌,周荣兴,李晓东,杨铁翼,郭军,吕慧成..长期应用糖皮质激素对大鼠股骨头骨组织MMPs/TIMPs系统的影响[J].西安交通大学学报（医学版）,2011,32(6):754-757,4.基金项目
国家自然科学基金资助项目(No.81160228) （No.81160228）
